Western Blot (WB)
((0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PPP1R15A/GADD34 Polyclonal Antibody | anti-PPP1R15A antibody
Goat anti-PPP1R15A/GADD34 Antibody
Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 75kDa band observed in lysates of cell line HEPG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.1-0.3ug/ml.
Note: We have established that some low non-specific signals as seen in WB make this antibody unfit for ICC/IF purposes.
Western Blot (WB)
((0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]
Uniprot Description
GADD34: Recruits the serine/threonine-protein phosphatase PP1 to dephosphorylate the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Belongs to the PPP1R15 family.
Protein type: DNA repair, damage; Translation initiation; Cell cycle regulation; Apoptosis
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; membrane; mitochondrial outer membrane; mitochondrion; protein phosphatase type 1 complex
Molecular Function: protein binding; protein kinase binding; protein phosphatase 1 binding; protein phosphatase type 1 regulator activity
Biological Process: aging; apoptosis; cell cycle arrest; negative regulation of cell proliferation; negative regulation of phosphoprotein phosphatase activity; negative regulation of protein amino acid dephosphorylation; positive regulation of apoptosis; positive regulation of phosphoprotein phosphatase activity; positive regulation of protein amino acid phosphorylation; positive regulation of translation initiation in response to stress; regulation of angiogenesis; response to cocaine; response to DNA damage stimulus; unfolded protein response
Research Articles on PPP1R15A
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Product Notes
The PPP1R15A ppp1r15a (Catalog #AAA420052) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PPP1R15A/GADD34 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R15A/GADD34 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration, 2-4ug/ml. Western Blot: Approx 75kDa band observed in lysates of cell line HEPG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.1-0.3ug/ml. Note: We have established that some low non-specific signals as seen in WB make this antibody unfit for ICC/IF purposes. Researchers should empirically determine the suitability of the PPP1R15A ppp1r15a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AAALDLSGRR G. It is sometimes possible for the material contained within the vial of "PPP1R15A/GADD34, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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