Rabbit GABARAP Polyclonal Antibody | anti-GABARAP antibody

Anti-GABARAP Antibody

Cat. Num. AAA417026

• Gene Names: GABARAP; MM46; ATG8A; GABARAP-a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: GABARAP; Polyclonal Antibody; Anti-GABARAP Antibody; Anti-GABRB1 (Ab-434) Antibody; anti-GABARAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GABRB1 (Ab-434) antibody detects endogenous levels of total GABRB1 protein.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 117

• Applicable Applications for anti-GABARAP antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Storage Buffer: Rabbit IgG in phosphate buffered saline (without Mg< sup>2< /sup>+ and Ca< sup>2< /sup>+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Immunogen: The antibody was produced against synthesized non-phosphopeptide derived from human GABRB1 around the phosphorylation site of serine 434 (R-A-SP-Q-L).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-GABARAP antibody

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 6005764
• NCBI GeneID: 11337
• NCBI Accession #: NP_009209.1
• NCBI GenBank Nucleotide #: NM_007278.1
• Molecular Weight: 13,918 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor-associated protein
• NCBI Official Synonym Full Names: GABA(A) receptor-associated protein
• NCBI Official Symbol: GABARAP
• NCBI Official Synonym Symbols: MM46; ATG8A; GABARAP-a
• NCBI Protein Information: gamma-aminobutyric acid receptor-associated protein
• UniProt Protein Name: Gamma-aminobutyric acid receptor-associated protein
• Protein Family: Gamma-aminobutyric acid receptor-associated protein
• UniProt Gene Name: GABARAP
• UniProt Entry Name: GBRAP_HUMAN

NCBI Description

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq, Jul 2008]

Uniprot Description

GABARAP: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Belongs to the MAP1 LC3 family. Interacts with GPHN and NSF. Interacts with GABRG2, beta-tubulin and ULK1.

Protein type: Adaptor/scaffold; Vesicle; Ubiquitin-like modifier; Autophagy; Microtubule-binding

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: microtubule; smooth endoplasmic reticulum; lysosome; autophagic vacuole; cytosol; actin cytoskeleton; Golgi membrane; microtubule associated complex; extrinsic to membrane; perinuclear region of cytoplasm; plasma membrane; cytoplasmic vesicle; axoneme

Molecular Function: protein binding; microtubule binding; beta-tubulin binding; GABA receptor binding

Biological Process: synaptic transmission; induction of apoptosis via death domain receptors; mitochondrion degradation; microtubule cytoskeleton organization and biogenesis; protein targeting; autophagic vacuole formation