Rabbit CUL2 Polyclonal Antibody | anti-CUL2 antibody

Anti-CUL2 Antibody

Cat. Num. AAA416445

• Reactivity: Human, Mouse, Rat. Not yet tested in other species.
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: CUL2; Polyclonal Antibody; Anti-CUL2 Antibody; anti-CUL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat. Not yet tested in other species.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 758

• Applicable Applications for anti-CUL2 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA titer: 1:5000-1:20,000; Immunohistochemistry: 1:100-500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal CUL2 antibody was raised against a peptide from intermediate residues of human CUL2 protein
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Immunohistochemistry (IHC)

References

(1) Maeda,Y., Suzuki,T., Pan,X., et al.CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis. J. Biol. Chem. 283 (23), 16084-16092 (2008)

NCBI and Uniprot Product Information

• NCBI GI #: 311771641
• NCBI GeneID: 8453
• NCBI Accession #: NP_001185708.1
• NCBI GenBank Nucleotide #: NM_001198779.1
• Molecular Weight: 89,492 Da
• NCBI Official Full Name: cullin-2 isoform b
• NCBI Official Synonym Full Names: cullin 2
• NCBI Official Symbol: CUL2
• NCBI Protein Information: cullin-2; CUL-2
• UniProt Protein Name: Cullin-2
• Protein Family: Cullin
• UniProt Gene Name: CUL2
• UniProt Entry Name: CUL2_HUMAN

Uniprot Description

CUL2: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Belongs to the cullin family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: nucleoplasm; Cul2-RING ubiquitin ligase complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; viral reproduction; protein ubiquitination; cell cycle arrest; G1/S transition of mitotic cell cycle