Rabbit ASB4 Polyclonal Antibody | anti-ASB4 antibody

Anti-ASB4 Antibody

Cat. Num. AAA416245

• Gene Names: ASB4; ASB-4
• Reactivity: Human. Predicted: Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: ASB4; Polyclonal Antibody; Anti-ASB4 Antibody; anti-ASB4 antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1151

• Applicable Applications for anti-ASB4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:1,000-1:2,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal ASB4 (1) antibody was raised against a recombinated human ASB4 protein 164-337aa (BC113479).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ASB4 antibody

This gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but some of the full length sequences are not known. [provided by RefSeq, Jul 2008]

References

(1) Ferguson,J.E. III, Wu,Y., Smith,K., et al. ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism. Mol. Cell. Biol. 27 (18), 6407-6419 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 109731278
• NCBI GeneID: 51666
• Molecular Weight: 38,931 Da
• NCBI Official Full Name: Homo sapiens ankyrin repeat and SOCS box-containing 4, mRNA
• NCBI Official Synonym Full Names: ankyrin repeat and SOCS box containing 4
• NCBI Official Symbol: ASB4
• NCBI Official Synonym Symbols: ASB-4
• NCBI Protein Information: ankyrin repeat and SOCS box protein 4; ankyrin repeat and SOCS box-containing 4
• UniProt Protein Name: Ankyrin repeat and SOCS box protein 4
• Protein Family: Ankyrin repeat and SOCS box protein
• UniProt Gene Name: ASB4
• UniProt Entry Name: ASB4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but some of the full length sequences are not known. [provided by RefSeq, Jul 2008]

Uniprot Description

ASB4: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 7q21-q22

Cellular Component: Cul2-RING ubiquitin ligase complex; Cul5-RING ubiquitin ligase complex

Molecular Function: ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: protein autoubiquitination