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Western Blot (WB)

Rabbit PISD Polyclonal Antibody | anti-PISD antibody

Anti-PISD Antibody

Gene Names
PISD; PSD; PSDC; PSSC; DJ858B16; dJ858B16.2
Reactivity
Human. Predicted: Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
PISD; Polyclonal Antibody; Anti-PISD Antibody; anti-PISD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human. Predicted: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
2693
Applicable Applications for anti-PISD antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
ELISA titer: 1:20,000-1:80,000
Western blot analysis: 1:500-1:1,000
Immunohistochemistry: 1:100-1:500
Optimal dilutions/concentrations should be determined by the end user
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
raised against a recombinate human PISD protein 71-310aa (BC001482)
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Western Blot (WB)

Immunohistochemistry (IHC) #2

Immunohistochemistry (IHC) #2
Related Product Information for anti-PISD antibody
Lyophilized PISD antibody purified by protein affinity column containing 1% BSA and 0.02% NaN3
References
(1) Hendrickson,S.L., Lautenberger,J.A., Chinn,L.W., et al. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. PLoS ONE 5 (9), E12862 (2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
43,047 Da
NCBI Official Full Name
Homo sapiens phosphatidylserine decarboxylase, mRNA
NCBI Official Synonym Full Names
phosphatidylserine decarboxylase
NCBI Official Symbol
PISD
NCBI Official Synonym Symbols
PSD; PSDC; PSSC; DJ858B16; dJ858B16.2
NCBI Protein Information
phosphatidylserine decarboxylase proenzyme
UniProt Protein Name
Phosphatidylserine decarboxylase proenzyme
UniProt Gene Name
PISD
UniProt Entry Name
PISD_HUMAN

NCBI Description

Phosphatidylserine decarboxylases (PSDs; EC 4.1.1.65) catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits (Schuiki and Daum, 2009 [PubMed 19165886]).[supplied by OMIM, Jul 2010]

Uniprot Description

PISD: Belongs to the phosphatidylserine decarboxylase family. Heterodimer. 2 isoforms of the human protein are produced by alternative splicing

Protein type: Endoplasmic reticulum; EC 4.1.1.65; Lipid Metabolism - glycerophospholipid; Lyase

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: mitochondrion; nucleus

Molecular Function: phosphatidylserine decarboxylase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process

Research Articles on PISD

Similar Products

Product Notes

The PISD pisd (Catalog #AAA416149) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PISD Antibody reacts with Human. Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PISD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ELISA titer: 1:20,000-1:80,000 Western blot analysis: 1:500-1:1,000 Immunohistochemistry: 1:100-1:500 Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the PISD pisd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PISD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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