IL36B active protein

Recombinant Human IL36B

Cat. Num. AAA4159856

• Gene Names: IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL36B; Recombinant Human IL36B; FIL1 eta; Interleukin-1 eta; Interleukin-1 family member 8; Interleukin-1 homolog 2; IL36B active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS (with BSA as a carrier protein)
• Sequence Length: 164

• Bioactivity: IL-36 beta can bind to human IL-1 Rrp2 Fc Chimera with a linear range of 0.15-5 ug/mL as determined by its binding ability in a functional ELISA
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: Reconstitute in sterile PBS at 100ug/mL (containing at least 0.1% human or bovine serum albumin)
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for IL36B active protein

IL36B, also known as Interleukin 36 beta and IL1F8, is a cytokine belonging to the IL-1 family. It is actively secreted and is found to form a cytokine gene cluster on chromosome 2 with eight other IL1 cytokines. IL36B signals through the IL1Rrp2/IL-36R receptor on dendritic cells which then activates NFkB and MAPK signalling pathways to create a pro-inflammatory response. It stimulates the production of mature adipocytes, and IL6 and IL8 synovial fibroblasts. It also induces expression of some antimicrobial peptides and matrix metalloproteases. It is highly expressed in psoriatic plaques, as part of the IL36 signalling system is found in many epithelial barriers.

NCBI and Uniprot Product Information

• NCBI GI #: 20070252
• NCBI GeneID: 27177
• NCBI Accession #: NP_055253.2
• NCBI GenBank Nucleotide #: NP_055253.2
• UniProt Accession #: Q9NZH7
• Molecular Weight: 17.5 kDa
• NCBI Official Full Name: interleukin-36 beta isoform 1
• NCBI Official Synonym Full Names: interleukin 36 beta
• NCBI Official Symbol: IL36B
• NCBI Official Synonym Symbols: FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
• NCBI Protein Information: interleukin-36 beta
• UniProt Protein Name: Interleukin-36 beta
• Protein Family: Interleukin
• UniProt Gene Name: IL36B
• UniProt Synonym Gene Names: IL1F8; IL1H2; IL-1 eta; IL-1F8; IL-1H2
• UniProt Entry Name: IL36B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

IL36B: Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of T cell differentiation; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; immune response; inflammatory response