Rabbit anti-Human JMY Polyclonal Antibody | anti-JMY antibody

Anti-JMY Antibody

Cat. Num. AAA415958

• Gene Names: JMY; WHDC1L3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: JMY; Polyclonal Antibody; Anti-JMY Antibody; anti-JMY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 2128

• Applicable Applications for anti-JMY antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:500-1:1,000; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Raised against a recombinate human JMY protein 163-341aa (BC130624).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-JMY antibody

Rabbit polyclonal JMY

References

(1) Coutts,A.S., Weston,L. and La Thangue,N.B. A transcription co-factor integrates cell adhesion and motility with the p53 response Proc. Natl. Acad. Sci. U.S.A. 106 (47), 19872-19877 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 120659967
• NCBI GeneID: 133746
• Molecular Weight: 110,280 Da
• NCBI Official Full Name: Homo sapiens junction mediating and regulatory protein, p53 cofactor, mRNA
• NCBI Official Synonym Full Names: junction mediating and regulatory protein, p53 cofactor
• NCBI Official Symbol: JMY
• NCBI Official Synonym Symbols: WHDC1L3
• NCBI Protein Information: junction-mediating and -regulatory protein; WAS protein homology region 2 domain containing 1-like 3; junction-mediating and regulatory protein
• UniProt Protein Name: Junction-mediating and -regulatory protein
• Protein Family: Junction-mediating and -regulatory protein
• UniProt Gene Name: JMY
• UniProt Entry Name: JMY_HUMAN

Uniprot Description

JMY: Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. Belongs to the JMY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: nucleoplasm; cytoskeleton; leading edge; cytoplasm; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; transcription coactivator activity; actin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of transcription factor activity; DNA repair; cell cycle arrest