Rabbit anti-Human Orai1 Polyclonal Antibody | anti-Orai1 antibody

Anti-Human Orai1 (Extracellular) Antibody

Cat. Num. AAA4159156

• Gene Names: ORAI1; IMD9; TAM2; ORAT1; CRACM1; TMEM142A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified On Immobilized Antigen.
• Synonyms: Orai1; Polyclonal Antibody; Anti-Human Orai1 (Extracellular) Antibody; Orai; anti-Orai1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Canis identical, bovine-12/13.
• Purity/Purification: Affinity Purified On Immobilized Antigen.
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 287

• Applicable Applications for anti-Orai1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: Jurkat (T lymphocyte, acute T cell leukemia) and HL-60 (human promyelocytic leukemia) cell line lysates (1:200).; Indirect Flow Cytometry: HL-60 and Jurkat live cells.
• Immunogen: Peptide (C)KKQPGQPRPTSK corresponding to residues of 203-214 of human Orai1 (Accession Q96D31).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-Orai1 antibody

Cytosolic calcium (Ca+2) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1, 2 The mechanism controlling intracellular Ca+2 level influx either by the calcium-release-activated Ca+2 channels (CRAC), or from intracellular stores, has become of great interest. Recently, several key players of the store operated complex have been identified.3 Orai1 (also known as CRACM1) acts as the store operated calcium channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca+2 sensor.3, 4 The formation of functional channels by Orai1 requires the presence of both Orai1 and STIM1 proteins working as a complex that involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum.4-6 TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex.7 In T-cells, Ca+2 entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit.3, 8 Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N-and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai3.3, 9 All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes.9 Alomone Labs is pleased to offer a new antibody raised against an extracellular epitope of the human Orai1 channel, Anti-Orai1(extracellular) antibody

NCBI and Uniprot Product Information

• NCBI GI #: 75771974
• NCBI GeneID: 84876
• NCBI Official Full Name: ORAI1 protein, partial
• NCBI Official Synonym Full Names: ORAI calcium release-activated calcium modulator 1
• NCBI Official Symbol: ORAI1
• NCBI Official Synonym Symbols: IMD9; TAM2; ORAT1; CRACM1; TMEM142A
• NCBI Protein Information: calcium release-activated calcium channel protein 1

NCBI Description

The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]