Mouse anti-Human Tissue Factor Monoclonal Antibody | anti-F3 antibody

Anti-Human Tissue Factor Biotinylated Purified IgG Antibody

Cat. Num. AAA4157087

• Gene Names: F3; TF; TFA; CD142
• Reactivity: Human
• Purity: Purified
• Synonyms: Tissue Factor; Monoclonal Antibody; Anti-Human Tissue Factor Biotinylated Purified IgG Antibody; anti-F3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: Prior to conjugation, this antibody was specific for tissue factor as demonstrated by ELISA.
• Purity/Purification: Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 295

• Application Notes: Suitable as a source of biotinylated antibodies to human tissue factor.
• Immunogen: Recombinant human tissue factor.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Related Product Information for anti-F3 antibody

Tissue Factor (TF) is an integral membrane glycoprotein expressed in the plasma membranes of many cell types. It is a single chain molecule of 44 kDa consisting of an extra-cellular domain (residues 1-219), a trans-membrane domain (residues 220-242) and the Cterminal intracellular domain of residues 243-263. Most abundant in the tissue adventitia, TF becomes exposed to blood at the site of vascular injury. The availability of TF is important in initiating coagulation by acting as a receptor for both the zymogen and protease forms of plasma factor VII (FVII and FVIIa), as well as mediating the conversion of bound FVII to FVIIa. The binding of FVII to TF in the presence of a negatively charged surface such as a phospholipid (or cell surface) promotes the auto activation of FVII by FVIIa. The TF-FVIIa complex in the presence of calcium ions proteolytically activates factors IX and X. These enzyme products_recover are then capable of activating FVII to FVIIa by feedback amplification. The activity of TF-FVIIa activity is regulated by a TFPI (tissue factor pathway inhibitor), a member of the Kunin superfamily of protease inhibitors. TFPI contains three kunitz domains and is able to bind and inhibit the TF-FVIIa complex in the presence of activated factor X and calcium ions. Antithrombin has also been reported to inhibit FVIIa activity in the presence of TF and heparin. Although a membrane protein, low levels of TF products_recover have been demonstrated in plasma. Increased levels of circulating TF products_recover may be a risk factor for thrombotic disease 1-4.

NCBI and Uniprot Product Information

• NCBI GI #: 339508
• NCBI GeneID: 2152
• NCBI Official Full Name: tissue factor
• NCBI Official Synonym Full Names: coagulation factor III, tissue factor
• NCBI Official Symbol: F3
• NCBI Official Synonym Symbols: TF; TFA; CD142
• NCBI Protein Information: tissue factor
• Protein Family: Tissue factor

NCBI Description

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]