Rabbit anti-Human, Rat WEE1 Polyclonal Antibody | anti-WEE1 antibody

Anti-WEE1 Antibody

Cat. Num. AAA415512

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: WEE1; Polyclonal Antibody; Anti-WEE1 Antibody; anti-WEE1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 3374

• Applicable Applications for anti-WEE1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA titer: 1:80,000; Western blot analysis: 1:200-500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-WEE1 antibody

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

References

(1) Igarashi,M., Nagata,A., Jinno,S., Suto,K. and Okayama,H. Wee1(+)-like gene in human cells.Nature 1991;353 (6339): 80-83.

NCBI and Uniprot Product Information

• NCBI GI #: 221307497
• NCBI GeneID: 7465
• NCBI Accession #: NP_003381.1
• NCBI GenBank Nucleotide #: NM_003390
• Molecular Weight: 49,072 Da
• NCBI Official Full Name: Homo sapiens WEE1 G2 checkpoint kinase (WEE1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: WEE1 G2 checkpoint kinase
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase; WEE1 homolog; wee1A kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Entry Name: WEE1_HUMAN

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.10.2; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; cell division; establishment of cell polarity; regulation of cell cycle; mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; G2/M transition of mitotic cell cycle; blood coagulation; neurite morphogenesis; G1/S transition of mitotic cell cycle