Growth hormone Active Protein
Rainbow Trout (Oncorhynchus mykiss) Growth Hormone (GH-1/GHP-35)
Purity
Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Synonyms
Growth hormone; Rainbow Trout (Oncorhynchus mykiss) Growth Hormone (GH-1/GHP-35); Growth hormone active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel.
Form/Format
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.5% NaHCO3 adjusted to pH 8.
Sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Glu-Asn-Gln-Arg.
Physical Appearance
White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized rtGH in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Dimers and Aggregates
The purified rtGH (21K) consists of > 99% monomers as determined by gel-filtration chromatography.
Biological Activity
rtGH is biologically active in PDF-P1 3B9 cells stably transfected with rabbit GH receptors, though its activity is ~ 10-fold lower than that of human GH.
Endotoxin
Less than 0.05 ng/µg (IEU/µg) of rtGH (22 K).
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.64 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the DNAman program.
Preparation and Storage
Lyophilized rtGH although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 mg/ml and filter sterilization rtGH can be stored at 4C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Related Product Information for Growth hormone active protein
Recombinant rainbow trout growth hormone (rtGH) produced in E. coli (22 K) is a single, non-glycosylated, polypeptide chain containing 188 amino acids with an additional Ala at its N-terminus and having a molecular mass of 21535 Dalton. rtGH is expressed as inclusion bodies, refolded and purified by proprietary chromatographic techniques.
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Product Notes
The Growth hormone (Catalog #AAA400131) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Gl u-Asn-Gln- Arg. It is sometimes possible for the material contained within the vial of "Growth hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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