IGF-I Active Protein | hIGF-1 active protein

Human IGF-I

• Gene Names: IGF1; IGF-1; IGF-I
• Purity: Greater than 98.0% as determined by:; (a) Analysis by reducing and non-reducing SDS-PAGE gel.; (b) Gel-filtration chromatography under non denaturing conditions.
• Synonyms: IGF-I; Human IGF-I; Human insulin-like growth factor 1 (hIGF-1); hIGF-1 active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by reducing and non-reducing SDS-PAGE gel.; (b) Gel-filtration chromatography under non denaturing conditions.
• Form/Format: White lyophilized (freeze-dried) powder.
• Sequence: MGPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
• Sequence Length: 154

• Solubility: It is recommended to reconstitute the lyophilized hIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.58 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
• Dimers and Aggregates: The purified hIGF-I consists of > 97% monomers as determined by gel-filtration chromatography.
• Endotoxin: Endotoxin level as measured by is <0.1EU/ug.
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
• Biological Activity: hIGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose -dependent proliferation of mouse BALBC 3T3 cells is less then 1.0 ng/ml.
• Preparation and Storage: Lyophilized hIGF-I although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 hIGF-I mg/ml and up to 2 2 mg/ml and filter sterilization gsIGF-I can be stored at +4 degree C.

Related Product Information for hIGF-1 active protein

IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant Human IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7655 Dalton.

Product Categories/Family for hIGF-1 active protein

Hormones; Cytokines and Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 118150782
• NCBI GeneID: 281239
• NCBI Accession #: NP_001071296.1
• NCBI GenBank Nucleotide #: NM_001077828.1
• UniProt Accession #: P07455
• Molecular Weight: 17,066 Da
• NCBI Official Full Name: insulin-like growth factor I preproprotein
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-1; IGF-I
• NCBI Protein Information: insulin-like growth factor I; class 1 insulin-like growth factor I preproprotein; insulin growth factor-1; insulin like growth factor 1; insulin-like growth factor I (somatomedin C); insulin-like growth factor I variant 1; insulin-like growth factor-1; prepro-IGF-I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-I
• UniProt Entry Name: IGF1_BOVIN

Uniprot Description

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().