growth hormone Active Protein | oGH active protein

Ovine growth hormone

Cat. Num. AAA400048

• Gene Names: GH1; GH; GH-N
• Purity: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Synonyms: growth hormone; Ovine growth hormone; Ovine pituitary growth hormone (oGH); oGH active protein

• Host: Escherichia Coli
• Purity/Purification: Greater than 98.0% as determined by:; (a) Analysis by RP-HPLC.; (b) Gel filtration at non denaturing conditions using 25 nM Tris-HCl + 150 nM NaCl, pH 8.; (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Form/Format: Sterile-filtered white lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.
• Sequence Length: 217

• Solubility: It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280 nm using the absorbency value of 0.684 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a calibrated solution of GH-22K-pl as a Reference Standard.
• Dimers and Aggregates: Less than 4% as determined by gel filtration chromatography
• Endotoxin: Less than 0.1 ng/ug (IEU/ug) of ovine GH.
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 8-9.
• Biological Activity: oGH is fully biologically active when compared to World Health Organization (WHO) reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD).
• Preparation and Storage: Lyophilized oGH although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution and filter sterilization oGH can be stored at 4 degree C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for oGH active protein

Recombinant ovine GH-22K produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 21833 Dalton. Predicted pI=7.81. oGH is purified by proprietary chromatographic techniques.

Product Categories/Family for oGH active protein

Growth Hormones; prolactins; placental lactogens

NCBI and Uniprot Product Information

• NCBI GI #: 374638325
• NCBI GeneID: 454774
• UniProt Accession #: P58756
• Molecular Weight: 24,843 Da
• NCBI Official Full Name: pituitary growth hormone
• NCBI Official Symbol: GH1
• NCBI Official Synonym Symbols: GH; GH-N
• NCBI Protein Information: somatotropin; Pituitary growth hormone
• UniProt Protein Name: Somatotropin
• Protein Family: Growth hormone
• UniProt Gene Name: GH1
• UniProt Synonym Gene Names: GH; GH-N
• UniProt Entry Name: SOMA_PANTR

Uniprot Description

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues ().