Rabbit Collagen Type I Antibody | anti-COL1A1 antibody
Collagen Type I, chicken
Applications
ELISA, Functional Assay, Immunofluorescence, Immunohistochemistry, immunoassay, Radioimmunoassay, Western Blot
Synonyms
Collagen Type I; chicken; anti-COL1A1 antibody
Host
Rabbit
Form/Format
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Concentration
1 mg/ml (varies by lot)
Sequence Length
1453
Applicable Applications for anti-COL1A1 antibody
ELISA, Immunofluorescence Assay (IFA), Immunohistochemistry - Paraffin (IHC-P), Radioimmunoassay (RIA), Western Blotting (WB)
Application Notes
Incubation Time: IHC(P) 60 min at RT or 2-8°C over night
Working Concentration: (purified, lyophilized) IFA - 1:80, IHC(P) - 1:1000, ELISA - 1:200 (OD - 500)
Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Positive Control: Chicken skin
Working Concentration: (purified, lyophilized) IFA - 1:80, IHC(P) - 1:1000, ELISA - 1:200 (OD - 500)
Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Positive Control: Chicken skin
Immunogen
Purified collagen type I from chicken skin
Secondary Reagents
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC or anti-rabbit IgG:DyLight488. (Please Inquire)
Preparation and Storage
Store at -20 degree C.
Related Product Information for anti-COL1A1 antibody
Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type I 100%, chicken collagen type II, III and V <0.1%; chicken fibronectin <0.1% RIA at 1:1000 dilution.
References
1. Mauger A., Demarchez M., Herbage D., Grimaud J.A., Druguet M., Hartmann D., Sengel P. (1982) Immunofluorescent localization of collagen types I and III, and of fibronectin during feather morphogenesis in the chick embryo. Dev. Biol. 94, 93-105. 2. Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol. 105, 999-1006. 3. Robert J., Hartmann D.J., Sengel P. (1989) Production of fibronectin and collagen types I and III by chick embryo dermal cells cultured on extracellular matrix substrates. Int. J. Dev. Biol. 33, 267-275. 4. Farjanel J., Hartmann D.J., Guidet B., Luquel L., Offenstadt G. (1993) Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome. Am. Rev. Respir. DIs. 147, 1091-1099.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Collagen alpha-1(I) chain
NCBI Official Synonym Full Names
collagen type I alpha 1 chain
NCBI Official Symbol
COL1A1
NCBI Protein Information
collagen alpha-1(I) chain
UniProt Protein Name
Collagen alpha-1(I) chain
Protein Family
UniProt Gene Name
COL1A1
Uniprot Description
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Research Articles on COL1A1
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Product Notes
The COL1A1 col1a1 (Catalog #AAA397115) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Collagen Type I can be used in a range of immunoassay formats including, but not limited to, ELISA, Immunofluorescence Assay (IFA), Immunohistochemistry - Paraffin (IHC-P), Radioimmunoassay (RIA), Western Blotting (WB). Incubation Time: IHC(P) 60 min at RT or 2-8°C over night Working Concentration: (purified, lyophilized) IFA - 1:80, IHC(P) - 1:1000, ELISA - 1:200 (OD - 500) Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Chicken skin. Researchers should empirically determine the suitability of the COL1A1 col1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen Type I, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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