Rabbit Collagen Type I Antibody | anti-COL1A1 antibody

Collagen Type I, human

Cat. Num. AAA397112

• Gene Names: COL1A1; OI1; OI2; OI3; OI4; EDSC; EDSARTH1
• Reactivity: Human, Cattle (<1.0%), Rat, Chicken (< 0.1%)
• Applications: Immunohistochemistry, Immunofluorescence, ELISA, immunoassay, Radioimmunoassay, Immunoblot, Western Blot, Western Blot
• Purity: Affinity Purified
• Synonyms: Collagen Type I; human; anti-COL1A1 antibody

• Host: Rabbit
• Reactivity: Human, Cattle (<1.0%), Rat, Chicken (< 0.1%)
• Purity/Purification: Affinity Purified
• Form/Format: Lyophilized from phosphate buffered solution; no BSA and preservative added.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1464

• Applicable Applications for anti-COL1A1 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA), Radioimmunoassay (RIA), Immunoblotting (IB), Western Blot (WB)
• Application Notes: IF: 1:80; IHC-P: 1:200-1:600; ELISA: >1:200 (OD>500); RIA: 1:500
• Immunogen: Purified collagen type I from human skin
• Positive Control: Human skin
• Incubation Time: IHC(P) 60 min at RT or 2-8 degree C over night
• Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
• Preparation and Storage: Store at -20 degree C.

Related Product Information for anti-COL1A1 antibody

Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type I, cross-reactivity with collagen type II and IV <0.1%, collagen type III and V <1.0%, human fibronectin and laminin <0.1%, mouse laminin <0.1% (RIA at a 1:500 dilution).

References

1. Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N. (1980) Collagen immunotyping in human liver: Light and electron microscope study. J. Histochem. Cytochem. 28, 1145-1156. 2. Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C. (1982) Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 74, 319-328. 3. Savino W., Itoh T., Imhof B.A., Dardenne M. (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus 8, 245-256. 4. Bedossa P., Bacci J., Lemaigre G., Martin E. (1987) Effects of fixation and processing on the immunohistochemical visualisation of type-I, -III and -IV collagen in paraffin-embedded liver tissue. Histochemistry 88, 85-89.

NCBI and Uniprot Product Information

• NCBI GI #: 110349772
• NCBI GeneID: 1277
• NCBI Accession #: NP_000079.2
• NCBI GenBank Nucleotide #: NM_000088.3
• UniProt Accession #: P02452; O76045; P78441; Q13896; Q13902; Q13903; Q14037; Q14992; Q15176; Q15201; Q16050; Q59F64
• Molecular Weight: 138,941 Da
• NCBI Official Full Name: collagen alpha-1(I) chain preproprotein
• NCBI Official Synonym Full Names: collagen type I alpha 1 chain
• NCBI Official Symbol: COL1A1
• NCBI Official Synonym Symbols: OI1; OI2; OI3; OI4; EDSC; EDSARTH1
• NCBI Protein Information: collagen alpha-1(I) chain
• UniProt Protein Name: Collagen alpha-1(I) chain
• Protein Family: Collagen
• UniProt Gene Name: COL1A1

NCBI Description

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Uniprot Description

Type I collagen is a member of group I collagen (fibrillar forming collagen).