Rabbit anti-Human CCR5 (HIV and chemokine receptor) Polyclonal Antibody | anti-CCR5 antibody

CCR5 (NT) (HIV and chemokine receptor)

Cat. Num. AAA395453

• Gene Names: CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: CCR5 (HIV and chemokine receptor); Polyclonal Antibody; CCR5 (NT) (HIV and chemokine receptor); HIV-1 fusion coreceptor; CHEMR13; CD195; C-C chemokine receptor type 5; anti-CCR5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative
• Sequence Length: 352

• Applicable Applications for anti-CCR5 antibody: Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to amino acids 6 to 20 of human CCR5.
• Positive Control: THP-1 whole cell lysates
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis using anti-CCR5 (NT) antibody at 0.5 ug/ml on THP-1 whole cell lysates.)

Related Product Information for anti-CCR5 antibody

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors1,2. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells3,4. CCR5 is required for the infection by HIV-1, HIV-2, and SIV1,2. The -chemokines RANTES, MIP-1 and MIP-1 are the ligands for CCR5 and prevent infection by M-tropic HIV-13-5. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells6,7. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites8. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

Product Categories/Family for anti-CCR5 antibody

Polyclonal Antibody; Chemokines & Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4502639
• NCBI GeneID: 1234
• NCBI Accession #: NP_000570.1
• NCBI GenBank Nucleotide #: NM_000579.3
• UniProt Accession #: P51681; O14692; O14693; O14695; O14696; O14697; O14698; O14699; O14700; O14701; O14702; O14703
• Molecular Weight: 40
• NCBI Official Full Name: C-C chemokine receptor type 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 5 (gene/pseudogene)
• NCBI Official Symbol: CCR5
• NCBI Official Synonym Symbols: CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
• NCBI Protein Information: C-C chemokine receptor type 5; chemr13; HIV-1 fusion coreceptor; chemokine receptor CCR5; C-C motif chemokine receptor 5 A159A
• UniProt Protein Name: C-C chemokine receptor type 5
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR5
• UniProt Synonym Gene Names: CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5
• UniProt Entry Name: CCR5_HUMAN

NCBI Description

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. Ref.1 Ref.2 Ref.3 Ref.11 Ref.12 Ref.16

Subunit structure: Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Interacts with human cytomegalovirus/HHV-5 protein UL78. Ref.2 Ref.3 Ref.13 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22

Subcellular location: Cell membrane; Multi-pass membrane protein Ref.16.

Tissue specificity: Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. Ref.1 Ref.2

Post-translational modification: Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen. Ref.14 Ref.18Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry. Ref.16Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.

Polymorphism: Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1) [

MIM:609423]. Variations in CCR5 gene also influence the rate of progression to AIDS after infection.Ser-60 variant, a naturally occurring mutation in a conserved residue in the first intracellular domain of CCR5, results in reduced amounts of the protein in the membrane and consequently may be associated with reduced susceptibility to infection by microbes that depend on these molecules as their receptors.Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection [

MIM:610379].

Involvement in disease: Diabetes mellitus, insulin-dependent, 22 (IDDM22) [MIM:612522]: A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical features are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.29

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.