Rabbit anti-Human FHL2 Polyclonal Antibody | anti-FHL2 antibody

FHL2

Cat. Num. AAA395405

• Gene Names: FHL2; DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: FHL2; Polyclonal Antibody; Four-and-a-half LIM only protein 2; anti-FHL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as ligand affinity purified antibody in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 279

• Applicable Applications for anti-FHL2 antibody: Western Blot (WB)
• Immunogen: Synthetic peptide derived from the human FHL2 protein
• Positive Control: Antibody has been tested on HeLa cell lysates.
• Preparation and Storage: Product should be stored at -70 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot using X1857P, rabbit polyclonal at 0.5 ug/ml on HeLa cell extract (10 ug/lane). Blots were developed with goat anti-rabbit Ig (1:75k) and Pierce?s Supersignal West Femto system.)

Related Product Information for anti-FHL2 antibody

Sphingosine kinase-1 (SK1) is a key enzyme that catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). SK1, and its product S1P, regulate numerous and diverse biological functions, including cell growth, differentiation, proliferation, and apoptosis. S1P also plays a central role in cardiac development and ischemic preconditioning. FHL2 (SLIM3) has been identified as a SK1-interacting protein in mammalian cardiomyocytes. FHL2, but not FHL1 or FHL3, interacts with SK1, and FHL2 colocalizes with SK1 in the cytoplasm of these cells. The interaction of FHL2 with SK-1 involves the C-terminal portion of SK1. Overexpression of FHL2 has been found to attneuate the activity and antiapoptotic effects of SK1. Endothelin-1, a potent survival factor in cardiomyocytes, inhibits FHL2-SK1 association and increases SK1 activity. FHL2 is therefore a novel inhibitor of SK1 activity in cardiomyocytes and targeting FHL2 may prevent myocardial apoptosis through activation of SK1.

Product Categories/Family for anti-FHL2 antibody

Antigen Immunoaffinity Purified Polyclonal; Growth Factors & Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 48146991
• NCBI GeneID: 2274
• UniProt Accession #: Q6I9R8; Q561W1; Q14192
• Molecular Weight: 32193 Da
• NCBI Official Full Name: FHL2
• NCBI Official Synonym Full Names: four and a half LIM domains 2
• NCBI Official Symbol: FHL2
• NCBI Official Synonym Symbols: DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• NCBI Protein Information: four and a half LIM domains protein 2; LIM domain protein DRAL; aging-associated gene 11; skeletal muscle LIM-protein 3; down-regulated in rhabdomyosarcoma LIM protein
• UniProt Protein Name: FHL2 protein
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL2
• UniProt Entry Name: Q6I9R8_HUMAN

NCBI Description

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

Sequence similarities: Contains 4 LIM zinc-binding domains. RuleBase RU000350