Mouse anti-Human Keratin 13 & 10 Monoclonal Antibody | anti-KRT13 antibody

Keratin 13 & 10

Cat. Num. AAA395320

• Gene Names: KRT13; K13; CK13
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A/G Chromatography
• Synonyms: Keratin 13 & 10; Monoclonal Antibody; anti-KRT13 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: DE-K13
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 420

• Applicable Applications for anti-KRT13 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from HeLa cells and mouse myeloma cells.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-KRT13 antibody

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Product Categories/Family for anti-KRT13 antibody

Monoclonal Antibody; Structural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 131412228
• NCBI GeneID: 3860
• NCBI Accession #: NP_002265.2
• NCBI GenBank Nucleotide #: NM_002274.3
• UniProt Accession #: P13646; Q53G54; Q6AZK5; Q8N240
• Molecular Weight: 53/56.6
• NCBI Official Full Name: keratin, type I cytoskeletal 13 isoform b
• NCBI Official Synonym Full Names: keratin 13
• NCBI Official Symbol: KRT13
• NCBI Official Synonym Symbols: K13; CK13
• NCBI Protein Information: keratin, type I cytoskeletal 13; CK-13; keratin-13; cytokeratin 13; cytokeratin-13
• UniProt Protein Name: Keratin, type I cytoskeletal 13
• Protein Family: Keratin
• UniProt Gene Name: KRT13
• UniProt Synonym Gene Names: CK-13; K13
• UniProt Entry Name: K1C13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Subunit structure: Heterotetramer of two type I and two type II keratins. keratin-13 is generally associated with keratin-4.

Tissue specificity: Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta. Ref.3

Post-translational modification: O-glycosylated; glycans consist of single N-acetylglucosamine residues. Ref.10

Involvement in disease: White sponge nevus of cannon (WSN) [MIM:193900]: Rare autosomal dominant disorder which predominantly affects non-cornified stratified squamous epithelia. Clinically, it is characterized by the presence of soft, white, and spongy plaques in the oral mucosa. The characteristic histopathologic features are epithelial thickening, parakeratosis, and vacuolization of the suprabasal layer of oral epithelial keratinocytes. Less frequently the mucous membranes of the nose, esophagus, genitalia and rectum are involved.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.15 Ref.16 Ref.17 Ref.18

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.