Western Blot/Blotting
(Lane 1. Western blot using anti-NPDC-1 on 10 ug lysate transfected with NPDC-1 protein at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate, 15 second exposure. Lane 2 probed with anti-Rem and anti-alpha SNAP antibodies.)
Mouse NPDC-1 Monoclonal Antibody | anti-NPDC-1 antibody
NPDC-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
NPDC-1; Monoclonal Antibody; anti-NPDC-1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
16E4
Form/Format
20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, 3mM sodium azide
Sequence Length
325
Applicable Applications for anti-NPDC-1 antibody
Western Blot (WB)
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide derived from human NPDC-1 protein and mouse myeloma cells.
Positive Control
Brain tissue lysate (mouse and rat tested, human not yet tested).
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Western Blot/Blotting
(Lane 1. Western blot using anti-NPDC-1 on 10 ug lysate transfected with NPDC-1 protein at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate, 15 second exposure. Lane 2 probed with anti-Rem and anti-alpha SNAP antibodies.)
Western Blot/Blotting
(Lane 1. Western blot using anti-NPDC-1 on 10 ug lysate transfected with NPDC-1 protein at a dilution of 1ug/ml, detected with Supersignal West Pico Substrate, 15 second exposure. Lane 2 probed with anti-Rem and anti-alpha SNAP antibodies.)
Related Product Information for anti-NPDC-1 antibody
The discovery of NPDC-1 (neuronal proliferation, differentiation and control protein 1) was the result of a study of genes that were preferentially expressed in immortalized neural precursor cell lines at the onset of contact inhibition of proliferation and subsequent differentiation. It was further found that transfection of transformed cells with NPDC-1 led to increased doubling times and suppression of many characteristics of transformation. NPDC-1 is differentially expressed in brain and lung and has been shown to interact directly with the transcription factor E2F-1 as well as D-type cyclins and cdk2. NPDC-1 has been identified as a modulator of transcriptiona levents mediated by retinoic acid and the protein level and activity of NPDC-1 have been shown to be regulated by phosphorylation mediated proteasomal degradation.
Product Categories/Family for anti-NPDC-1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NPDC-1 (Catalog #AAA395295) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NPDC-1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPDC-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NPDC-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPDC-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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