Mouse anti-Human Keratin 7 Monoclonal Antibody | anti-KRT7 antibody

Keratin 7

Cat. Num. AAA395242

• Gene Names: KRT7; K7; CK7; SCL; K2C7
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A/G Chromatography
• Synonyms: Keratin 7; Monoclonal Antibody; anti-KRT7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C35
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 469

• Applicable Applications for anti-KRT7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with purified human keratin 7 isolated from HeLa cytoskeleton and mouse myeloma cells.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-KRT7 antibody

Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

Product Categories/Family for anti-KRT7 antibody

Monoclonal Antibody; Structural Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 67782365
• NCBI GeneID: 3855
• NCBI Accession #: NP_005547.3
• NCBI GenBank Nucleotide #: NM_005556.3
• UniProt Accession #: P08729; Q92676; Q9BUD8; Q9Y3R7
• Molecular Weight: 51,386 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 7
• NCBI Official Synonym Full Names: keratin 7
• NCBI Official Symbol: KRT7
• NCBI Official Synonym Symbols: K7; CK7; SCL; K2C7
• NCBI Protein Information: keratin, type II cytoskeletal 7; CK-7; keratin-7; sarcolectin; cytokeratin 7; cytokeratin-7; type-II keratin Kb7; type II mesothelial keratin K7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7
• UniProt Protein Name: Keratin, type II cytoskeletal 7
• Protein Family: Keratin
• UniProt Gene Name: KRT7
• UniProt Synonym Gene Names: SCL; CK-7; K7
• UniProt Entry Name: K2C7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Ref.3 Ref.9

Subunit structure: Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7. Ref.8 Ref.9

Subcellular location: Cytoplasm Ref.2.

Tissue specificity: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. Ref.1 Ref.4

Induction: Up-regulated by retinoic acid. Ref.2

Post-translational modification: Arg-20 is dimethylated, probably to asymmetric dimethylarginine. Ref.7

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.

Mass spectrometry: Molecular mass is 51203.48 Da from positions 2 - 469. Determined by MALDI. Ref.10

Sequence caution: The sequence CAA26956.2 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.