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Western Blot/Blotting (Western blot using X1841M anti AFp monoclonal antibody at 4 ug/ml on HepG2 cell lysate (10 ug/lane). Anti-Mouse HRp was used at 1:75k. Blots were developed using Pierce?s Super Signal West Femto. Exposure was 1 2 minutes.)

Mouse alpha-Fetoprotein Monoclonal Antibody | anti-AFP antibody

alpha-Fetoprotein

Reactivity
Human, Dog, Pig
Applications
Immunohistochemistry
Purity
Protein A/G Chromatography
Synonyms
alpha-Fetoprotein; Monoclonal Antibody; AFP; anti-AFP antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Dog, Pig
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
C3
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
610
Applicable Applications for anti-AFP antibody
Immunohistochemistry (IHC)
Immunogen
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified human alpha-fetoprotein and mouse myeloma cells.
Positive Control
HepG2 cells
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot using X1841M anti AFp monoclonal antibody at 4 ug/ml on HepG2 cell lysate (10 ug/lane). Anti-Mouse HRp was used at 1:75k. Blots were developed using Pierce?s Super Signal West Femto. Exposure was 1 2 minutes.)

Western Blot/Blotting (Western blot using X1841M anti AFp monoclonal antibody at 4 ug/ml on HepG2 cell lysate (10 ug/lane). Anti-Mouse HRp was used at 1:75k. Blots were developed using Pierce?s Super Signal West Femto. Exposure was 1 2 minutes.)
Related Product Information for anti-AFP antibody
Alpha-Feto Protein (AFP) is a protein produced by the fetus, which is excreted into the amniotic fluid and into the mother's bloodstream through the placenta. The amount of AFP, both in the maternal?s blood and in the amniotic fluid, at particular periods during the pregnancy, may be associated with the presence of neural tube defects or chromosomal problems in the baby. AFP synthesis occurs in tissues including the gut, liver and fetal yolk sac. AFP expression may be seen in germ cell neoplasma and in liver carcinoma.
Product Categories/Family for anti-AFP antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,625 Da
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Protein Information
alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
Protein Family
UniProt Gene Name
AFP
UniProt Entry Name
FETA_PIG

Uniprot Description

Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin

By similarity.

Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form

By similarity.

Subcellular location: Secreted

By similarity.

Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.

Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.

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Product Notes

The AFP afp (Catalog #AAA395211) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The alpha-Fetoprotein reacts with Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's alpha-Fetoprotein can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-Fetoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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