Testing Data
(Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.)
Mouse anti-Mouse, Rat bcl -xl Monoclonal Antibody | anti-bcl -xl antibody
bcl -xl
Testing Data
(Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.)
Testing Data
(Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Potent inhibitor of cell death. Inhibits activation of caspases
By similarity. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Ref.17 Ref.19Isoform Bcl-X(S) promotes apoptosis. Ref.17 Ref.19
Subunit structure: Homodimer. Isoform Bcl-X(L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BCL2L11. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters
By similarity. Interacts with BAD. Interacts (isoform Bcl-X(L)) with SIVA1 (isoform 1); the interaction inhibits the anti-apoptotic activity. Interacts with BECN1 and PGAM5. Interacts (isoform Bcl-X(L)) with BAX (isoform Sigma). Isoform Bcl-X(L) interacts with IKZF3. Interacts with HEBP2. Isoform Bcl-X(L) interacts with BOP/C22orf29. Interacts with P53/TP53 and BBC3; interaction with BBC3 disrupts the interaction with P53/TP53. Ref.2 Ref.9 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.25 Ref.28 Ref.29
Subcellular location: Mitochondrion membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side
By similarity. Cytoplasm › cytoskeleton › centrosome. Note: Mitochondrial membranes and perinuclear envelope
By similarity. Localizes to the centrosome when phosphorylated at Ser-49. Ref.17 Ref.19
Tissue specificity: Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Domain: The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Post-translational modification: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity. Ref.11Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis. Ref.17 Ref.19
Sequence similarities: Belongs to the Bcl-2 family.
Research Articles on bcl -xl
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Product Notes
The bcl -xl bcl2l1 (Catalog #AAA395033) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The bcl -xl reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's bcl -xl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the bcl -xl bcl2l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "bcl -xl, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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