SDS-Page
(Recombinant UHRF1 protein gel 8% SDS-PAGE Coomassie staining MW: 93.1 kDa Purity: > 60%)
UHRF1 recombinant protein
Recombinant UHRF1 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant UHRF1 protein gel 8% SDS-PAGE Coomassie staining MW: 93.1 kDa Purity: > 60%)
SDS-Page
(Recombinant UHRF1 protein gel 8% SDS-PAGE Coomassie staining MW: 93.1 kDa Purity: > 60%)
Testing Data
(UHRF1 binding assay1 uM H3K9me3 peptide was incubated with different concentrations of UHRF1 protein in 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-His antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(UHRF1 binding assay1 uM H3K9me3 peptide was incubated with different concentrations of UHRF1 protein in 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-His antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
Testing Data
Background: UHRF1 (Ubiquitin Like With PHD And Ring Finger Domains 1, also known as Np95, hNP95, ICBP90, RNF106, TDRD22, hUHRF1, huNp95) is a member of a subfamily of RING-finger type E3 ubiquitin ligases. UHRF1 binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. UHTR1 plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. UHRF1 is overexpressed in various cancers. UHRF1 plays a critical role in maintaining DNA methylation in mammalian cells.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
Research Articles on UHRF1
Similar Products
Product Notes
The UHRF1 uhrf1 (Catalog #AAA389077) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "UHRF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
