Mouse BubR1 Monoclonal Antibody | anti-BubR1 antibody
BubR1 antibody (mAb)(Clone 8G1)
Background: BubR1 (also known as BUB1B, MAD3L and SSK1) is an essential component of the mitotic spindle integrity checkpoint and is required for the normal progression of mitosis, and thus can act as a tumor suppressor. It encodes a serine/threonine kinase localized to the kinetochore and is involved in spindle checkpoint function. BubR1 plays a role in the inhibition of the anaphase-promoting complex (APC), delaying the onset of anaphase and ensuring proper chromosome segregation. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle and the chromosomes are aligned on the metaphase plate. Another function involves the monitoring of kinetochore activities that depend on the kinetochore motor CENP-E. It is also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. BubR1 is autophosphorylated, and BubR1 kinase activity is stimulated by association with the centromere protein CENP-E. Phosphorylation at Ser670 and Ser1043 occurs at kinetochores upon mitotic entry with dephosphorylation occurring at the onset of anaphase. Impaired spindle checkpoint function has been found in many forms of cancer. Defects in BubR1 are involved in mosaic variegated aneuploidy syndrome (MVA) and premature chromatid separation trait (PCS). BubR1 interacts with CENP-E, CENP-F, mitosin, PLK1, CASC5, BUB3 and the anaphase-promoting complex (APC). It is part of a complex containing BUB3 and CDC2.
NCBI and Uniprot Product Information
Uniprot Description
BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; BUB family
Chromosomal Location of Human Ortholog: 15q15
Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition
Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait
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Product Notes
The BubR1 bub1b (Catalog #AAA388563) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BubR1 antibody (mAb)(Clone 8G1) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's BubR1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the BubR1 bub1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BubR1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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