Western Blot (WB)
(MRG15 pAb tested by Western blot. Nuclear extract of HeLa cells (20 ug) was probed with MRG15 pAb (1:500 dilution).)
Rabbit MRG15 Polyclonal Antibody | anti-MRG15 antibody
MRG15 antibody (pAb)
WB: 1:250-1:1,000 dilution
Western Blot (WB)
(MRG15 pAb tested by Western blot. Nuclear extract of HeLa cells (20 ug) was probed with MRG15 pAb (1:500 dilution).)
Western Blot (WB)
(MRG15 pAb tested by Western blot. Nuclear extract of HeLa cells (20 ug) was probed with MRG15 pAb (1:500 dilution).)
Background: MRG15 (MORF4-Related Gene on chromosome 15) is the homolog of the yeast Eaf3 protein and belongs to the MRG family of genes, which are involved in various cellular processes as cellular aging, positive and negative transcriptional regulation and DNA damage repair. MRG15 can be associated with both histone deacetylase and histone acetyltransferase complexes (such as the NuA4 histone acetyltransferase complex, which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal Histone H2A and Histone H4). MRG15, thus, potentially plays a role in both transcriptional silencing and activation. In addition to the more conserved MRG motifs, MRG15 also contains a chromodomain near the N-terminus.
NCBI and Uniprot Product Information
Uniprot Description
MORF4L1: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; Sin3 complex
Molecular Function: protein binding; protein N-terminus binding; chromatin binding
Biological Process: cell proliferation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of growth; histone deacetylation; double-strand break repair via homologous recombination
Research Articles on MRG15
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Product Notes
The MRG15 morf4l1 (Catalog #AAA388456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRG15 antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's MRG15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:250-1:1,000 dilution. Researchers should empirically determine the suitability of the MRG15 morf4l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRG15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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