SDS-Page
(Recombinant ALKBH5 protein gel Recombinant ALKBH5 was run on a 10% SDS-PAGE gel stained with Coomassie Blue. MW: 45.5 kDa Purity: > 90%)
ALKBH5 recombinant protein
Recombinant ALKBH5 protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant ALKBH5 protein gel Recombinant ALKBH5 was run on a 10% SDS-PAGE gel stained with Coomassie Blue. MW: 45.5 kDa Purity: > 90%)
SDS-Page
(Recombinant ALKBH5 protein gel Recombinant ALKBH5 was run on a 10% SDS-PAGE gel stained with Coomassie Blue. MW: 45.5 kDa Purity: > 90%)
Dot Blot (DB)
(Dot blot for Recombinant ALKBH5 protein activity. 3 uM m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with different concentrations of ALKBH5 proteins in a 30 ul reaction system including 50 mM HEPES-NaOH pH 7.5, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Then each reaction was concentrated to a final volume of 10 ul. 1 ul of each reaction was spotted onto a positively charged nylon membrane and products were detected by Dot blot assay with m6A antibody .)
Dot Blot (DB)
(Dot blot for Recombinant ALKBH5 protein activity. 3 uM m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with different concentrations of ALKBH5 proteins in a 30 ul reaction system including 50 mM HEPES-NaOH pH 7.5, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Then each reaction was concentrated to a final volume of 10 ul. 1 ul of each reaction was spotted onto a positively charged nylon membrane and products were detected by Dot blot assay with m6A antibody .)
Background: N6-methylated adenine (m6A) is prevalent in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also appears in other RNA molecules, such as primary miRNA (pri-miRNAs). ALKBH5 (AlkB Homolog 5, RNA Demethylase, also known as OFOXD1, ABH5) specifically demethylates m6A RNA. ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles via demethylation activity.
NCBI and Uniprot Product Information
Uniprot Description
ALKBH5: a protein contaning a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily domain. May be involved in DNA alkylation damage repair
Protein type: DNA repair, damage; EC 1.14.11.-; Membrane protein, integral; Oxidoreductase; RNA-binding
Chromosomal Location of Human Ortholog: 17p11.2|17p11.2
Cellular Component: cytosol; Golgi apparatus; nuclear speck; nucleoplasm; nucleus
Molecular Function: metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; RNA binding
Biological Process: cell differentiation; DNA dealkylation involved in DNA repair; mRNA export from nucleus; mRNA processing; response to hypoxia; spermatogenesis
Research Articles on ALKBH5
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Product Notes
The ALKBH5 alkbh5 (Catalog #AAA388291) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ALKBH5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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