METTL3 recombinant protein

Recombinant METTL3 protein

Cat. Num. AAA388270

• Gene Names: METTL3; M6A; IME4; Spo8; MT-A70
• Synonyms: METTL3; Recombinant METTL3 protein; Methyltransferase Like 3; MRNA (2-O-Methyladenosine-N(6)-)-Methyltransferase; METTL3 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant METTL3 protein is supplied at a concentration of 0.35ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant METTL3 protein gel. Recombinant METTL3 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 65.5 kDa Purity: > 95%)

Related Product Information for METTL3 recombinant protein

Short Description: Recombinant METTL3 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_062826.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of METTL3 is 65.5 kDa. It is suitable for analysis of RNA methylation and modification, RNA splicing, processing and transcription regulation assays.

Background: N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs). METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.

Product Categories/Family for METTL3 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 21361827
• NCBI GeneID: 56339
• NCBI Accession #: NP_062826.2
• NCBI GenBank Nucleotide #: NM_019852.3
• UniProt Accession #: Q86U44; O14736; Q86V05; Q9HB32
• Molecular Weight: 25,479 Da
• NCBI Official Full Name: N6-adenosine-methyltransferase 70 kDa subunit
• NCBI Official Synonym Full Names: methyltransferase like 3
• NCBI Official Symbol: METTL3
• NCBI Official Synonym Symbols: M6A; IME4; Spo8; MT-A70
• NCBI Protein Information: N6-adenosine-methyltransferase 70 kDa subunit; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase
• UniProt Protein Name: N6-adenosine-methyltransferase 70 kDa subunit
• Protein Family: N6-adenosine-methyltransferase
• UniProt Gene Name: METTL3
• UniProt Synonym Gene Names: MTA70; MT-A70
• UniProt Entry Name: MTA70_HUMAN