SDS-Page
(Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag protein gel. SMARCA4 / BRG1 (1448-1569), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue.)
SMARCA4/BRG1 recombinant protein
Recombinant SMARCA4/BRG1 (1448-1569), GST-Tag
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag protein gel. SMARCA4 / BRG1 (1448-1569), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue.)
SDS-Page
(Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag protein gel. SMARCA4 / BRG1 (1448-1569), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue.)
Testing Data
(Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3 uM H3K14ac peptide was incubated with different concentrations of SMARCA4 / BRG1 (1448-1569) protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul Anti-GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3 uM H3K14ac peptide was incubated with different concentrations of SMARCA4 / BRG1 (1448-1569) protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul Anti-GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3 uM H3K14ac peptide was incubated with different concentrations of SMARCA4 / BRG1 (1448-1569) protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul Anti-GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3 uM H3K14ac peptide was incubated with different concentrations of SMARCA4 / BRG1 (1448-1569) protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul Anti-GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA4 contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA4 is part of the large ATP-dependent chromatin remodeling complex SNF/SWI which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Gene mutation causes Rhabdoid Tumor Predisposition Syndrome Type 2. SMARCA4 functions as a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It also interacts with glucocorticoid receptor (GR), TOPBP1 and progesterone receptor (PR) and is a component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Somatic mutations of SMARCA4 have been detected in some cancer cell lines and loss of SMARCA4 is associated with decreased survival in cancer patients.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Research Articles on SMARCA4/BRG1
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Product Notes
The SMARCA4/BRG1 (Catalog #AAA388193) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SMARCA4/BRG1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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