DNMT3B/DNMT3L recombinant protein

Recombinant DNMT3B/DNMT3L complex

Cat. Num. AAA388134

• Gene Names: DNMT3B; ICF; ICF1; M.HsaIIIB
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: DNMT3B/DNMT3L; Recombinant DNMT3B/DNMT3L complex; DNA Methyltransferase 3 Like; DNA Methyltransferase 3 Beta; DNMT3B/DNMT3L recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Full length recombinant DNMT3B/DNMT3L complex is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% Glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant DNMT3B / DNMT3L complex gel Recombinant DNMT3B / DNMT3L complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue. MW: DNMT3B: 89.7 kDa DNMT3L: 48.6 kDa Purity: > 91%)

Testing Data

(Recombinant DNMT3B / DNMT3L complex activity assay Assay was performed using DNMT Activity / Inhibition Assay. Add 100 ul reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubation was for 2 hour at 37 degree C.)

Related Product Information for DNMT3B/DNMT3L recombinant protein

Short Description: Recombinant DNMT3B/DNMT3L complex that includes full-length human DNMT3B protein (accession number NP_787045.1) with an N-terminal 6xHis-tag and DNMT3L (accession number NP_787063.1) with an N-terminal 6xHis-tag and DYKDDDDK-Tag was expressed in Sf9 cells. The molecular weights of DNMT3B and DNMT3L are 89.7 kDa and 48.6 kDa, respectively. The recombinant protein is >90% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: DNA methyltransferase proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. DNMT3L is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation.

Product Categories/Family for DNMT3B/DNMT3L recombinant protein

Recombinant Proteins; DNA Methyltransferase (DNMT)

NCBI and Uniprot Product Information

• NCBI GI #: 28559063
• NCBI GeneID: 1789
• NCBI Accession #: NP_787045.1; NP_787063.1
• NCBI GenBank Nucleotide #: NM_175849.1
• UniProt Accession #: Q9UBC3; Q9UBD4; Q9UJQ5; Q9UKA6; Q9UNE5; A2A2E2; B4DSM8; B4DSU1; E1P5M6; E1P5M7; E7EN63; E9PBF2
• Molecular Weight: 81,311 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3B isoform 3
• NCBI Official Synonym Full Names: DNA methyltransferase 3 beta
• NCBI Official Symbol: DNMT3B
• NCBI Official Synonym Symbols: ICF; ICF1; M.HsaIIIB
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3B
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3B
• UniProt Gene Name: DNMT3B
• UniProt Synonym Gene Names: Dnmt3b; DNA MTase HsaIIIB; M.HsaIIIB
• UniProt Entry Name: DNM3B_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]

Uniprot Description

DNMT3B: a ubiquitous DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Numerous cancer cell lines and primary acute leukemias express aberrant DNMT3B transcripts, especially DNMT3B7. Transfection of DNMT3B7 into 293 cells alters the pattern of genes expressed in the transfected cells. Can interact with DNMT1, which might be a co-operative event during DNA methylation. Methylates CpG sites at a rate slower than DNMT3A and much slower than DNMT1. Interacts with SUV39H1, SETDB1, SUMO1 and UBE2I9. Interacts with DNMT1 and DNMT3A. Mutations in DNMT3B have been shown to cause immunodeficiency-centromeric instability-facial anomalies syndrome (ICF). Six alternatively spliced isoforms of the human protein have been reported. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. Isoforms 4 and 5 are probably not enzymatically active due to the deletion of two conserved methyltransferase motifs.

Protein type: Methyltransferase; Methyltransferase, DNA; Cell development/differentiation; EC 2.1.1.37; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; unmethylated CpG binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; metal ion binding; DNA-methyltransferase activity; transcription corepressor activity

Biological Process: response to drug; negative regulation of histone H3-K9 methylation; genetic imprinting; S-adenosylhomocysteine metabolic process; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; cytosine methylation within a CG sequence; protein complex localization; methylation-dependent chromatin silencing; negative regulation of gene expression, epigenetic; DNA methylation; S-adenosylmethioninamine metabolic process; gene expression; response to ionizing radiation; positive regulation of neuron differentiation; DNA methylation on cytosine

Disease: Immunodeficiency-centromeric Instability-facial Anomalies Syndrome 1