Human Retinoblastoma Protein 1 ELISA Kit | RB1 elisa kit
Human Retinoblastoma Protein 1 ELISA Kit
Reactivity
Human
Synonyms
Retinoblastoma Protein 1; Human Retinoblastoma Protein 1 ELISA Kit; RB1 elisa kit
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human RB1 and analogues was observed.
Sequence Length
16
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
31.2 ng/ml - 1000 ng/ml.
Sensitivity
5.0 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RB1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RB1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RB1 and RB1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for RB1 elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RB1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RB1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RB1 and RB1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
retinoblastoma, partial
Protein Family
NCBI Description
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
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Product Notes
The Human RB1 (Catalog #AAA038165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA038165 ELISA Kit recognizes Human RB1. It is sometimes possible for the material contained within the vial of "Retinoblastoma Protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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