Mouse Acrosin ELISA Kit | ACR elisa kit

Mouse Acrosin ELISA Kit

Cat. Num. AAA3806426

• Reactivity: Mouse
• Synonyms: Acrosin; Mouse Acrosin ELISA Kit; ACR elisa kit

• Reactivity: Mouse
• Sequence Length: 421

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 100 pg/ml-1600 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACR elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofACROSINin the sample, This ACROSINELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusACROSINconcentration. The concentration ofACROSINin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148613878
• NCBI GeneID: 49
• NCBI Accession #: NP_001088
• NCBI GenBank Nucleotide #: NP_001088.2
• UniProt Accession #: P10323
• NCBI Official Full Name: acrosin
• NCBI Official Synonym Full Names: acrosin
• NCBI Official Symbol: ACR
• NCBI Protein Information: acrosin
• UniProt Protein Name: Acrosin
• Protein Family: Acrosin
• UniProt Gene Name: ACR
• UniProt Synonym Gene Names: ACRS
• UniProt Entry Name: ACRO_HUMAN

NCBI Description

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008]

Uniprot Description

ACR: Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.10

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: protein complex; extracellular region; Golgi-associated vesicle; nucleus; acrosomal matrix

Molecular Function: mannose binding; protein binding; copper ion binding; amidase activity; DNA binding; zinc ion binding; serine-type endopeptidase activity; drug binding; fucose binding

Biological Process: response to steroid hormone stimulus; binding of sperm to zona pellucida; single fertilization; acrosome reaction; adenylate cyclase activation; penetration of zona pellucida; acrosome matrix dispersal; multicellular organism reproduction