Human Splicing Factor 3B Subunit 3 ELISA Kit | SF3B3 elisa kit

Human Splicing Factor 3B Subunit 3 ELISA Kit

Cat. Num. AAA3803601

• Gene Names: SF3B3; RSE1; SAP130; SF3b130; STAF130
• Reactivity: Human
• Synonyms: Splicing Factor 3B Subunit 3; Human Splicing Factor 3B Subunit 3 ELISA Kit; SF3B3 elisa kit

• Reactivity: Human
• Sequence Length: 1217

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 250 pg/ml-4000 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SF3B3 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofSPLICING FACTOR 3B SUBUNIT 3in the sample, This SPLICING FACTOR 3B SUBUNIT 3ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusSPLICING FACTOR 3B SUBUNIT 3concentration. The concentration ofSPLICING FACTOR 3B SUBUNIT 3in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 54112121
• NCBI GeneID: 23450
• NCBI Accession #: NP_036558
• NCBI GenBank Nucleotide #: NP_036558.3
• UniProt Accession #: Q15393
• NCBI Official Full Name: splicing factor 3B subunit 3
• NCBI Official Synonym Full Names: splicing factor 3b subunit 3
• NCBI Official Symbol: SF3B3
• NCBI Official Synonym Symbols: RSE1; SAP130; SF3b130; STAF130
• NCBI Protein Information: splicing factor 3B subunit 3
• UniProt Protein Name: Splicing factor 3B subunit 3
• Protein Family: Splicing factor
• UniProt Gene Name: SF3B3
• UniProt Synonym Gene Names: KIAA0017; SAP130; SF3b130; SAP 130
• UniProt Entry Name: SF3B3_HUMAN

NCBI Description

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3B3: Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Belongs to the RSE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; U12-dependent spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; protein complex assembly; gene expression; mRNA processing