K2/Spice Synthetic Cannabinoids ELISA Kit

K2/Spice Synthetic Cannabinoids ELISA Assay Kit

Cat. Num. AAA376013

• Synonyms: K2/Spice Synthetic Cannabinoids; K2/Spice Synthetic Cannabinoids ELISA Assay Kit; K2/Spice Synthetic Cannabinoids elisa kit

• Samples: Urine, whole blood, serum, and plasma
• Assay Procedure: 1. Allow all kit components to equilibrate to room temperature. 2. Dilute test specimens with phosphate buffered saline to minimize matrix effects, if required. 3. Add 20uL of test samples (blanks, calibrators, quality control samples, and test specimens) to wells of the microplate as required by the user (see example plate set-up). 4. Add 100uL of JWH-018 conjugate to each well. Tap each side of the plate holder to ensure thorough mixing. 5. Incubate plate for 60 minutes at room temperature (20-25 degree C) with gentle agitation. 6. Wash the plate wells 5-times with 325uL of phosphate buffered saline using a multi-tip pipette or 4-times with an automated plate washer. Be careful that the pipette tips do not touch the well bottoms. 7. After washing, invert and gently tap the plate twice onto absorbent paper placed on the benchtop to remove residual liquid (not required if using an automated microplate washer). 8. Add 100ul TMB SUBSTRATE to each well. Tap each side of plate holder to ensure thorough mixing. 9. Incubate at room temp for 30 min with gentle agitation, preferably in the dark or dim light. The wells will develop colorless to blue in color. 10. Add 100uL STOP SOLUTION to each well. CAUTION: MILDLY CAUSTIC SOLUTION! AVOID CONTACT WITH SKIN AND EYES! The wells will immediately change to a yellow color. 11. Read OD at lambda450nm in a microplate reader between 5 to 30 min after STOP SOLUTION addition. Be careful to eliminate any air bubbles in the wells before reading. NOTE: The above suggested assay procedure can be modified to better suit the users current laboratory equipment and set-up. In all circumstances, the user must validate any method for suitability of their intended purpose.
• Preparation of Experimental Samples: Prepare phosphate buffered saline solution by completely dissolving each packet into 1 liter dH2O. Dilute test samples in phosphate buffered saline to minimize matrix effects, if required. Typically, human urine samples can be used directly. User must determine appropriate dilutions for test samples.
• Preparation and Storage: The kit components are stable for at least 3 months from date of shipment when stored at 4 degree C (see lot specific expiration date). Place unused microwell strips back into the sealed pouch containing desiccant. Do not use components from different lot shipments. Note: Unopened phosphate buffered saline packet, #K205, is stable indefinitely and can be used with any kit lots.; Calibrator standards at -20 or -70 degree C. Specimen and quality control samples (e.g.: urine, plasma) at -20 or -70 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for K2/Spice Synthetic Cannabinoids elisa kit

Product Description: K2/Spice Synthetic Cannabinoids ELISA Assay Kit is a 96-well microplate assay designed to test the presence of JWH-018, JWH-073, JWH-122, JWH-019, JWH-081, AM-2201 and related compounds and their metabolites in samples such as urine, whole blood, serum, and plasma. Using the positive control compound JWH-018 N-5-hydroxypentyl metabolite analog, the limit of detection in urine is 5ng/mL. Total assay time is approximately 2 hours. This assay does not cross-react significantly with JWH-250, UR-144, or XLR-11.