Mouse hnRNP C1+C2 Monoclonal Antibody | anti-hnRNP C1+C2 antibody

Mouse Anti-hnRNP C1+C2 [4F4]

Cat. Num. AAA375142

• Gene Names: HNRNPC; C1; C2; HNRNP; HNRPC; SNRPC
• Reactivity: Chicken, Hamster, Human, Monkey
• Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
• Purity: Protein A
• Synonyms: hnRNP C1+C2; Monoclonal Antibody; Mouse Anti-hnRNP C1+C2 [4F4]; C1 antibody; C2 antibody; Heterogeneous nuclear ribonucleoprotein C antibody; Heterogeneous nuclearribonucleoproteins C1/C2 antibody; HNRNP antibody; hnRNP C1 / hnRNP C2 antibody; hnRNP C1/C2 antibody; hnRNPCantibody; HNRPC antibody; HNRPC_HUMAN antibody; MGC104306 antibody; MGC105117 antibody; MGC117353antibody; MGC131677 antibody; Nuclear ribonucleoprotein particle C1 protein antibody; Nuclear ribonucleoproteinparticle C2 protein antibody; SNRPC antibody; anti-hnRNP C1+C2 antibody

• Host: Mouse
• Reactivity: Chicken, Hamster, Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F4
• Specificity: This antibody recognizes the two hnRNP C proteins of 41kDa (C1) and 43kDa (C2) in human cells
• Purity/Purification: Protein A
• Form/Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 293

• Applicable Applications for anti-hnRNP C1+C2 antibody: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Optimal antibody dilution should be determined by titration.
• Target Antigen: hnRNP C1+C2
• Immunogen: RNPs eluted from oligo (dT) cellulose column
• Myeloma/fusion Partners: SP2/0
• Research Areas: RNA binding; RNA Processing
• Preparation and Storage: Storage at + 4degreee C for one month, or in small aliquots at -20 degree C for longer periods. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

References

De Wever V et al. Isolation of human mitotic protein phosphatase complexes: identification of a complex between protein phosphatase 1 and the RNA helicase Ddx21.PLoS One. 2012;7(6):e39510. doi: 10.1371/journal.pone.0039510. Epub 2012 Jun 28.WB ; HumanPMID: 22761809 Li JJ et al. Genomic instability of gold nanoparticle treated human lung fibroblast cells. Biomaterials 32:5515-23 (2011). WB ; Human PUBMED Ertel KJ et al. Mechanistic consequences of hnRNP C binding to both RNA termini of poliovirus negative-strand RNA intermediates. J Virol 84:4229-42 (2010). WB ; Human PUBMED Brunner JE et al. Delayed kinetics of poliovirus RNA synthesis in a human cell line with reduced levels of hnRNP C proteins. Virology 400:240-7 (2010). WB ; Human PUBMED Nicolas A et al. Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments. J Virol 84:8871-87 (2010). WB ; Human PUBMED Herrmann F et al. Human protein arginine methyltransferases in vivo--distinct properties of eight canonical members of the PRMT family. J Cell Sci 122:667-77 (2009). WB ; Human PUBMED Mosessian S et al. Analysis of PTEN complex assembly and identification of heterogeneous nuclear ribonucleoprotein C as a component of the PTEN-associated complex. J Biol Chem 284:30159-66 (2009). WB, ICC/IF ; Human PUBMED Ogawa S et al. Physical and functional interactions between STAT5 and Runx transcription factors.J Biochem. 2008 May;143(5):695-709. doi: 10.1093/jb/mvn022. Epub 2008 Feb 22.WB ; Human Hamster PMID: 18296717 Barrandon C et al. The transcription-dependent dissociation of P-TEFb-HEXIM1-7SK RNA relies upon formation of hnRNP-7SK RNA complexes. Mol Cell Biol 27:6996-7006 (2007). IP ; Human PUBMED Baird SD et al. A search for structurally similar cellular internal ribosome entry sites. Nucleic Acids Res 35:4664-77 (2007). WB ; Human PUBMED Kuyumcu-Martinez NM et al. Increased steady-state levels of CUGBP1 in myotonic dystrophy 1 are due to PKC-mediated hyperphosphorylation. Mol Cell 28:68-78 (2007). WB ; Human PUBMED Fu D & Collins K Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation. Mol Cell 28:773-85 (2007). IP ; Human PUBMED Tang LY et al. Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction.Mol Cell Proteomics. 2007 Nov;6(11):1952-67. Epub 2007 Aug 12.WB ; Human PMID: 17693683 Zhang D et al. Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study. Leuk Res. 2007 Jan;31(1):75-82. Epub 2006 Apr 17.WB ; Human PMID: 16616778 Lin YT & Yen PH A novel nucleocytoplasmic shuttling sequence of DAZAP1, a testis-abundant RNA-binding protein. RNA 12:1486-93 (2006) PUBMED Watson IR et al. Mdm2-mediated NEDD8 modification of TAp73 regulates its transactivation function. J Biol Chem 281:34096-103 (2006) PUBMED Isolation of the heterogeneous nuclear RNAribonucleoprotein complex (hnRNP): a unique supramolecular assembly. Choi Y.D et al (Proc Natl Acad Sci U S A,Dec 1984)PUBMED

NCBI and Uniprot Product Information

• NCBI GI #: 117190174
• NCBI GeneID: 3183
• NCBI Accession #: NP_004491.2
• NCBI GenBank Nucleotide #: NM_004500.3
• UniProt Accession #: P07910; P22628; Q53EX2; Q59FD3; Q5FWE8; Q86SF8; Q86U45; Q96HK7; Q96HM4; Q96IY5; D3DS19; D3DS22
• Molecular Weight: 27,822 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoproteins C1/C2 isoform b
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• NCBI Official Symbol: HNRNPC
• NCBI Official Synonym Symbols: C1; C2; HNRNP; HNRPC; SNRPC
• NCBI Protein Information: heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; nuclear ribonucleoprotein particle C1 protein; nuclear ribonucleoprotein particle C2 protein
• UniProt Protein Name: Heterogeneous nuclear ribonucleoproteins C1/C2
• UniProt Gene Name: HNRNPC
• UniProt Synonym Gene Names: HNRPC; hnRNP C1/C2
• UniProt Entry Name: HNRPC_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP C1/C2: a heterogeneous nuclear ribonucleoprotein. May play a role in ribonucleosome assembly by neutralizing basic proteins such as A and B core hnRNP. Contains 1 RNA recognition motif (RRM) domain. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; spliceosome; protein complex; membrane; nuclear chromatin; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; nucleosomal DNA binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; poly(U) binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; RNA splicing; ATP-dependent chromatin remodeling; gene expression