IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MCM3 antibody. Blue: DAPI for nuclear staining.)
Rabbit MCM3 Polyclonal Antibody | anti-MCM3 antibody
MCM3 Polyclonal Antibody
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human, Mouse, Monkey
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
MCM3, Antibody; MCM3 Polyclonal Antibody; MCM3; HCC5; MGC1157; P1-MCM3; P1.h; RLFB; anti-MCM3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
808
Applicable Applications for anti-MCM3 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human MCM3
Calculated Molecular Weight
91kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MCM3 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using MCM3 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MCM3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MCM3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-MCM3 antibody
The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).
Product Categories/Family for anti-MCM3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
808
NCBI Official Full Name
DNA replication licensing factor MCM3
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
NCBI Protein Information
DNA replication licensing factor MCM3; p102; RLF subunit beta; hRlf beta subunit; DNA replication factor MCM3; cervical cancer proto-oncogene 5; minichromosome maintenance deficient 3; replication licensing factor, beta subunit; MCM3 minichromosome maintenance deficient 3; DNA polymerase alpha holoenzyme-associated protein P1
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Gene Name
MCM3
UniProt Entry Name
MCM3_HUMAN
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Product Notes
The MCM3 mcm3 (Catalog #AAA37034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCM3 Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MCM3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MCM3 mcm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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