IL-3 recombinant protein

IL-3 Protein (Active)

Cat. Num. AAA355560

• Gene Names: IL3; IL-3; MCGF; MULTI-CSF
• Applications: SDS-Page
• Synonyms: IL-3; IL-3 Protein (Active); Interleukin-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3; IL-3 recombinant protein

• Form/Format: Lyophilized

• Applicable Applications for IL-3 recombinant protein: SDS-PAGE
• Dimers: < 1% by SDS-PAGE
• Endotoxin Level: < 0.1 ng/ug (1 EU/ug) by LAL test
• Reconstitution: Reconstitute with sterile 18MΩ-cm H2O.
• Storage Buffer: Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.
• Storage Buffer: Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.
• Preparation and Storage: Ship at 4 degree C. Upon receipt, store at -20 degree C or -80 degree C for 2 years. After reconstituting, it can store at 4 degree C for a week, it is recommended to add 0.1% HSA or BSA for long term. Avoid repeated freeze and thaw cycles.

Related Product Information for IL-3 recombinant protein

Recombinant human IL-3 protein with molecular weight 15 kDa.
Interleukin 3 (IL-3) is a hematopoietic colony-stimulating factor that is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. It can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL3 infusion expands an early cell population that subsequently requires the action of a later-acting factor such as GMCSF to complete its development.

References

1. Chavany, C., Vicario-Abejon, C., Miller, G., Jendoubi, M. Transgenic mice for interleukin 3 develop motor neuron degeneration associated with autoimmune reaction against spinal cord motor neurons. Proc. Nat. Acad. Sci. 95: 11354-11359, 1998. 2. Donahue, R. E., Seehra, J., Metzger, M., Lefebvre, D., Rock, B., Carbone, S., Nathan, D. G., Garnick, M., Sehgal, P. K., Laston, D., LaVallie, E., McCoy, J., Schendel, P. F., Norton, C., Turner, K., Yang, Y.-C., Clark, S. C. Human IL-3 and GM-CSF act synergistically in stimulating hematopoiesis in primates. Science 241: 1820-1823, 1988. 3. Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell 47 (1): 3-10.

NCBI and Uniprot Product Information

• NCBI GI #: 3002475
• NCBI GeneID: 3562
• UniProt Accession #: P08700; Q6GS87
• Molecular Weight: 17,233 Da
• NCBI Official Full Name: IL-3
• NCBI Official Synonym Full Names: interleukin 3 (colony-stimulating factor, multiple)
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF; MULTI-CSF
• NCBI Protein Information: interleukin-3; mast cell growth factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; hematopoietic growth factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor
• UniProt Protein Name: Interleukin-3
• UniProt Gene Name: IL3
• UniProt Synonym Gene Names: IL-3; MCGF
• UniProt Entry Name: IL3_HUMAN

NCBI Description

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Activated T-cells, mast cells, natural killer cells.

Sequence similarities: Belongs to the IL-3 family.