Human P-Selectin ELISA Kit | SELP elisa kit

P-Selectin Human ELISA Kit

Cat. Num. AAA355417

• Gene Names: SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• Reactivity: Human
• Applications: ELISA
• Synonyms: P-Selectin; P-Selectin Human ELISA Kit; Human P-Selectin; SELP elisa kit

• Reactivity: Human

• Applicable Applications for SELP elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of P-Selectin in serum, plasma, body fluids, tissue lysates or cell culture supernates.
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- P-Selectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- P-Selectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the P-Selectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of P-Selectin can be calculated.

Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule that in humans is encoded by the SELP gene. It is located on chromosome 1q21-q24, spans>50 kb and contains 17 exons in human. It is constitutively expressed on megakaryocytes (the precursor of platelets) and endothelial cells. P-selectin plays an essential role in the initial recruitment of leukocytes (white blood cells) to the site of injury during inflammation. Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase-resistant structures, clearly different than those reported for E-selectin, and suggest disparate roles for P-selectin and E-selectin during recruitment during inflammatory responses.

NCBI and Uniprot Product Information

• NCBI GI #: 157419154
• NCBI GeneID: 6403
• NCBI Accession #: NP_002996.2
• NCBI GenBank Nucleotide #: NM_003005.3
• UniProt Accession #: P16109; Q5R344; Q8IVD1
• Molecular Weight: 90,834 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin P (granule membrane protein 140kDa, antigen CD62)
• NCBI Official Symbol: SELP
• NCBI Official Synonym Symbols: CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• NCBI Protein Information: P-selectin; GMP-140; granule membrane protein 140; granulocyte membrane protein; CD62 antigen-like family member P; platelet alpha-granule membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMRP; GRMP; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_HUMAN

NCBI Description

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Ref.7

Subunit structure: Interacts with SNX17. Interacts with PSGL1/SEPL and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of PSGL1 and PODXL2, and specific tyrosine sulfation on PSGL1. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.12 Ref.15

Subcellular location: Membrane; Single-pass type I membrane protein.

Tissue specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Involvement in disease: Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.20

Sequence similarities: Belongs to the selectin/LECAM family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 9 Sushi (CCP/SCR) domains.