PTH [1-34] (Human) IgG fraction Polyclonal Antibody | anti-pth antibody

PTH [1-34] (Human) IgG fraction

Cat. Num. AAA352250

• Applications: Western Blot
• Synonyms: PTH [1-34] (Human) IgG fraction; Polyclonal Antibody; Parathyroid Hormone [1-34]; PTH [1-34] (Human); IgG Fraction of Rabbit Anti-hPTH (1-34) Serum; anti-pth antibody

• Clonality: Polyclonal
• Specificity: Polypeptide ::: % Cross Reactivity; PTH (1-34) (human) ::: 100; PTH (1-84) (human) ::: 100; PTH (1-84) (bovine) ::: 80; PTHrp (1-35) ::: < 0.1; ANP ::::::: 0; ACTH (1-39) (human)::::::: 0; VIP:::::: 0
• Form/Format: Each vial contains 2.24 mg of purified IgG; Lyophilized Powder

• Applicable Applications for anti-pth antibody: Immunocytochemical Staining, Western Blot
• Application Notes: Immunocytochemistry: This IgG fraction has been found to stain formaldehyde fixed sections known to express PTH at a concentration of 25 ug/ml.; ; Western Blot: 1. After SDS-PAGE on a 15% gel and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.; 2. Wash x 2 with TBS/Tween-20.; 3. Apply the IgG fraction after dilution to at least 25 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.; 4. Wash x 3 with TBS/Tween-20.; 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.; 6. Wash x 4 for 5 minute/wash with TBS/Tween-20.; 7. Develop color using the enhanced DAB reaction.
• Preparation and Storage: Store at 2°C to 8°C

Related Product Information for anti-pth antibody

This IgG fraction was prepared by precipitation, dialysis and chromatography from an antiserum that was raised in a rabbit which was immunized with hPTH(1-34) covalently attached onto a carrier protein. The antiserum has been shown to be specific for hPTH(1-34) and was characterized by western blotting, ELISA, RIA and cell staining techniques. This IgG fraction is suitable for immunocytochemical and western immunoblotting detection of hPTH. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent concentration in whole antiserum, or with additional buffer for more dilute antibody.

Product Categories/Family for anti-pth antibody

Histochemical Grade Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 222420251
• UniProt Accession #: B9DLD9
• NCBI Official Full Name: pth
• UniProt Protein Name: Peptidyl-tRNA hydrolase
• Protein Family: Parathyroid hormone
• UniProt Gene Name: pth
• UniProt Entry Name: PTH_STACT

Uniprot Description

Function: The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis

By similarity. HAMAP MF_00083

Catalytic activity: N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083

Subunit structure: Monomer

By similarity. HAMAP MF_00083

Subcellular location: Cytoplasm

By similarity HAMAP MF_00083.

Sequence similarities: Belongs to the PTH family.