Rabbit anti-Human heNOS myristylated Polyclonal Antibody
Rabbit Anti-human endothelial
Nitric Oxide Synthase (Myristylated form) Serum
Reactivity
Human
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
heNOS myristylated; Polyclonal Antibody; Rabbit Anti-human endothelialNitric Oxide Synthase (Myristylated form) Serum; anti-heNOS myristylated antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
heNOS (cap-2-11Cys12) ::: 100
recombinant myristylated heNOS (myristylated eNOS type III) ::: 100
heNOS (cap-2-11Cys12) ::: 100
recombinant myristylated heNOS (myristylated eNOS type III) ::: 100
Form/Format
Antiserum Specificity
Applicable Applications for anti-heNOS myristylated antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes
Immunocytochemistry: This antiserum has been found to stain fixed cultured cells known to express myristylated eNOS by indirect immunofluorescence at a dilutions of 1:400.
Western Blot: Western immunoblots using homogenate from cells expressing heNOS resulted in a single band being detected at ~ 135 kDa at a concentration of 25 ug/ml.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 7.5% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2 - 4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 min per wash cycle in a fairly large volume of TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Western Blot: Western immunoblots using homogenate from cells expressing heNOS resulted in a single band being detected at ~ 135 kDa at a concentration of 25 ug/ml.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 7.5% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2 - 4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 min per wash cycle in a fairly large volume of TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-heNOS myristylated antibody
This antiserum was raised in a rabbit which was immunized with heNOS (caproyl-2-11Cys12) covalently attached onto a carrier protein. This antiserum is specific for the myristylated form of hecNOS. It is suitable for immunocytochemical and western immunoblotting detection of myristylated heNOS. The antiserum has been characterized by western blotting. ELISA. and cell staining techniques using synthetic peptides and recombinant whole proteins. Dilute the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum.
Product Categories/Family for anti-heNOS myristylated antibody
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Product Notes
The heNOS myristylated (Catalog #AAA352105) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-human endothelial Nitric Oxide Synthase (Myristylated form) Serum reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's heNOS myristylated can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot. Immunocytochemistry: This antiserum has been found to stain fixed cultured cells known to express myristylated eNOS by indirect immunofluorescence at a dilutions of 1:400. Western Blot: Western immunoblots using homogenate from cells expressing heNOS resulted in a single band being detected at ~ 135 kDa at a concentration of 25 ug/ml. Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 7.5% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the rabbit polyclonal antibody after dilution to at least 1:500 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2 - 4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1 - 2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 min per wash cycle in a fairly large volume of TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the heNOS myristylated for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "heNOS myristylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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