D-3 Dopamine receptor IgG fraction Polyclonal Antibody
D-3 Dopamine receptor IgG fraction
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
D-3 Dopamine receptor IgG fraction; Polyclonal Antibody; D 3 (2-10); D 3 Dopamine Receptor; IgG Fraction of Rabbit Anti-D3 Receptor Sera; anti-D-3 Dopamine receptor IgG fraction antibody
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
D3 Dopamine Receptor (2-10) ::: 100
D3 Dopamine Receptor ::: 80
D3 Dopamine Receptor (2-10) ::: 100
D3 Dopamine Receptor ::: 80
Form/Format
Antiserum Specificity
Applicable Applications for anti-D-3 Dopamine receptor IgG fraction antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes
Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections at a concentration of 5 ug/ml. This includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactroy bulb..
Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~50 KDa at a concentration of 25 ug/ml dilution.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after dilution to at least 25 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~50 KDa at a concentration of 25 ug/ml dilution.
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after dilution to at least 25 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-D-3 Dopamine receptor IgG fraction antibody
The antiserum was raised in a rabbit which was immunized with D3(2-10-Cys11) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum and the IgG fraction of the antiserum have been found to be highly specific for this peptide sequence and are suitable for immunocytochemical detection of the D3 dopamine receptor. Rehydrate the lyophilized IgG fraction with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody. Each vial contains 1.88 mg of purified rabbit IgG.
Product Categories/Family for anti-D-3 Dopamine receptor IgG fraction antibody
Similar Products
Product Notes
The D-3 Dopamine receptor IgG fraction (Catalog #AAA352072) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's D-3 Dopamine receptor IgG fraction can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot. Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections at a concentration of 5 ug/ml. This includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactroy bulb. Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~50 KDa at a concentration of 25 ug/ml dilution. Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the IgG fraction after dilution to at least 25 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash x 4 for 5 minute/wash with TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the D-3 Dopamine receptor IgG fraction for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "D-3 Dopamine receptor IgG fraction, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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