Rabbit D-1 Dopamine receptor Polyclonal Antibody | anti-DRD1 antibody

Rabbit Anti-D1 Receptor Sera

Cat. Num. AAA352066

• Gene Names: DRD1; DADR; DRD1A
• Applications: Immunoblot, Western Blot, Western Blot
• Synonyms: D-1 Dopamine receptor; Polyclonal Antibody; Rabbit Anti-D1 Receptor Sera; D1 Receptor (9-21); D1 Dopamine Receptor; anti-DRD1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Polypeptide vs % Cross Reactivity; D1 Dopamine Receptor (9-21): 100; D1 Dopamine Receptor: 90; D2 Dopamine Receptor (272-282): 0; D2 Dopamine Receptor: 0; D3 Dopamine Receptor (2-10): 0; D3 Dopamine Receptor: 0; D4 Dopamine Receptor (176-185): 0; D4 Dopamine Receptor: 0; D5 Dopamine Receptor (23-35): 0; D5 Dopamine Receptor: 0
• Form/Format: Antiserum Specificity
• Sequence Length: 446

• Applicable Applications for anti-DRD1 antibody: Immunocytochemical Staining, Western Immunoblot, Western Blot
• Application Notes: Immunocytochemistry: This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:5000. This includes the Medial septum. Nucleus accumbens. Dentate gyrus. Globus pallidus. Medial forebrain bundle. Cortex regions 1-3. Substantia Nigra reticulata. and the Ventral tegmental area.; ; Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~50 kD at 1:1000 dilution.; ; Western Blot Protocol: 1. After SDS-PAGE on 12% gels and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.; 2. Wash x 2 with TBS/Tween-20.; 3. Apply the rabbit polyclonal antibody after dilution to at least 1:1000 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.; 4. Wash x 3 with TBS/Tween-20.; 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.; 6. Wash x 4 for 5 minute/wash with TBS/Tween-20.; 7. Develop color using the enhanced DAB reaction.
• Preparation and Storage: Store at 2 to 8 degree C.

Related Product Information for anti-DRD1 antibody

The antiserum was raised in a rabbit which was immunized with D1(Ac-9-21-Cys22) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum has been found to be highly specific for this peptide sequence and is suitable for immunocytochemical and western blot detection of the D1 dopamine receptor. Rehydrate the lyophilized antibody with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody

Product Categories/Family for anti-DRD1 antibody

Histochemical Grade Antibodies; Dopamine Receptor Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4503383
• NCBI GeneID: 1812
• NCBI Accession #: NP_000785.1
• NCBI GenBank Nucleotide #: NM_000794.3
• UniProt Accession #: P21728; Q4QRJ0; B2RA44; Q6FH34
• Molecular Weight: 49162 Da
• NCBI Official Full Name: D(1A) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

Uniprot Description

Function: This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit structure: Interacts with DNAJC14 via its C-terminus

By similarity. Interacts with DRD1IP.

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Endoplasmic reticulum membrane; Multi-pass membrane protein

By similarity. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14

Tissue specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Ref.1

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.