IL-13 elispot set

Human IL-13 ELISpot Pair

Cat. Num. AAA335782

• Gene Names: IL13; P600; IL-13
• Synonyms: IL-13; Human IL-13 ELISpot Pair; IL-13 elispot set

• Sequence Length: 146

• Assay Type: Sandwich

Related Product Information for IL-13 elispot set

Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and tranplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principle of the Assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

NCBI and Uniprot Product Information

• NCBI GI #: 26787978
• NCBI GeneID: 3596
• NCBI Accession #: NP_002179.2
• NCBI GenBank Nucleotide #: NM_002188.2
• Molecular Weight: 15,816 Da
• NCBI Official Full Name: interleukin-13
• NCBI Official Synonym Full Names: interleukin 13
• NCBI Official Symbol: IL13
• NCBI Official Synonym Symbols: P600; IL-13
• NCBI Protein Information: interleukin-13
• UniProt Protein Name: Interleukin-13
• UniProt Gene Name: IL13
• UniProt Synonym Gene Names: NC30; IL-13
• UniProt Entry Name: IL13_HUMAN

NCBI Description

This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]

Uniprot Description

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: cytoplasm; external side of plasma membrane; extracellular region; extracellular space

Molecular Function: cytokine activity; interleukin-13 receptor binding; protein binding

Biological Process: cell motility; cell-cell signaling; immune response; inflammatory response; microglial cell activation; negative regulation of NAD(P)H oxidase activity; positive regulation of B cell proliferation; positive regulation of connective tissue growth factor production; positive regulation of immunoglobulin production; positive regulation of macrophage activation; positive regulation of mast cell degranulation; positive regulation of protein secretion; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of smooth muscle cell proliferation; positive regulation of tyrosine phosphorylation of Stat6 protein; regulation of proton transport; response to ethanol; response to lipopolysaccharide; response to nicotine; signal transduction

Disease: Allergic Rhinitis; Asthma, Susceptibility To