Human Gastrin-Reliasing Peptide ELISA Kit | GRP elisa kit

Human Gastrin-Reliasing Peptide ELISA Kit

Cat. Num. AAA32206

• Gene Names: GAST; GAS
• Reactivity: Human
• Synonyms: Gastrin-Reliasing Peptide; Human Gastrin-Reliasing Peptide ELISA Kit; GRP elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human GRP and analogues was observed.
• Sequence Length: 101

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml - 100 pg/ml.
• Sensitivity: 1.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GRP concentrations in Human serum, plasma and other body fluids. Using Purified Human GRP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRP and GRP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GRP elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GRP concentrations in Human serum, plasma and other body fluids. Using Purified Human GRP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRP and GRP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503923
• NCBI GeneID: 2520
• NCBI Accession #: NP_000796.1
• NCBI GenBank Nucleotide #: NM_000805.4
• UniProt Accession #: P01350; P78463; P78464; AAB19304
• Molecular Weight: 11,394 Da
• NCBI Official Full Name: gastrin preproprotein
• NCBI Official Synonym Full Names: gastrin
• NCBI Official Symbol: GAST
• NCBI Official Synonym Symbols: GAS
• NCBI Protein Information: gastrin; preprogastrin
• UniProt Protein Name: Gastrin
• Protein Family: Gastrin-releasing peptide
• UniProt Gene Name: GAST
• UniProt Synonym Gene Names: GAS; G52; G34; G17; G14; G6
• UniProt Entry Name: GAST_HUMAN

NCBI Description

Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]

Uniprot Description

gastrin: Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine. Belongs to the gastrin/cholecystokinin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular region

Molecular Function: protein binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; signal transduction