anti-Human CD11b Monoclonal Antibody | anti-ITGAM antibody
MAb to CD11b
Gene Names
ITGAM; CD11B
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Synonyms
CD11b; Monoclonal Antibody; MAb to CD11b; Monoclonal Antibody to CD11b; gp 95-165kDa; anti-ITGAM antibody
Host
Mouse. Hybridization of Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
BEAR-1
Specificity
CD11b
Reacts with monocytes, polynuclear and null cells. The recognized structure is the alpha chain of a heterodimer glycoprotein of Mw 165kD (alpha chain) and 95kD (beta chain).
Reacts with monocytes, polynuclear and null cells. The recognized structure is the alpha chain of a heterodimer glycoprotein of Mw 165kD (alpha chain) and 95kD (beta chain).
Form/Format
Purified, Lyophilized.
Reconstitute with 1ml of distilled water.
Reconstitute with 1ml of distilled water.
Concentration
0.2mg/ml (prior to lyophilization) (varies by lot)
Applicable Applications for anti-ITGAM antibody
Flow Cytometry, Immunohistochemistry - Formalin
Application Notes
Fluorescence microscopy or flow cytometry: 2ug / 5x105 cells/test.
Immunohistochemistry: Working dilution: 1:25 to 1:50. Only suitable on cryostat sections or cell smears.
Each laboratory should determine an optimum working titer for use in its part
Immunohistochemistry: Working dilution: 1:25 to 1:50. Only suitable on cryostat sections or cell smears.
Each laboratory should determine an optimum working titer for use in its part
Immunogen
Purified human monocytes
Affinity Constant
Not determined
Buffer
Lyophilized from PBS containing 1mg/ml BSA
Preservative
No
Lyophilized
Yes
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Preparation and Storage
Store lyophilized product at 2 to 8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles. The addition of 0.1% (w/v) sodium azide is recommended for storage of the reconstituted form for up to one month at 2
Product Categories/Family for anti-ITGAM antibody
References
• Keizer, G., et al., (1985), "Biochemical and functional characteristics of the human leucocyte membrane antigen family LFA-1, MO-1", Eur. J. Immunol., 15, 1142.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ITGAM (Catalog #AAA311978) is an Antibody produced from Mouse. Hybridization of Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. and is intended for research purposes only. The product is available for immediate purchase. The MAb to CD11b reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry, Immunohistochemistry - Formalin. Fluorescence microscopy or flow cytometry: 2ug / 5x105 cells/test. Immunohistochemistry: Working dilution: 1:25 to 1:50. Only suitable on cryostat sections or cell smears. Each laboratory should determine an optimum working titer for use in its part. Researchers should empirically determine the suitability of the ITGAM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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