anti-Human Alpha Fetoprotein (AFP) Monoclonal Antibody | anti-AFP antibody

MAb to Alpha Fetoprotein (AFP)

Cat. Num. AAA311666

• Gene Names: AFP; FETA; HPAFP; AFP
• Reactivity: Human
• Applications: ELISA
• Purity: >90% pure (SDS-PAGE). Protein A chromatography
• Synonyms: Alpha Fetoprotein (AFP); Monoclonal Antibody; MAb to Alpha Fetoprotein (AFP); MAb to AFP; Monoclonal Antibody to Human Alpha Fetoprotein; anti-AFP antibody

• Host: Host: Mouse; Source: Ascites
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 057-11301
• Specificity: Alpha Fetoprotein (AFP); Human Alpha Fetoprotein (AFP)
• Purity/Purification: >90% pure (SDS-PAGE). Protein A chromatography
• Form/Format: Purified, Liquid
• Concentration: 4.71 mg/ml (OD280nm, E^0.1% = 1.4) (varies by lot)
• Sequence Length: 609

• Applicable Applications for anti-AFP antibody: ELISA; Immunoassay Antibody Pairs
• Immunogen: Human AFP
• Affinity Constant: Not determined
• Buffer: 10mM Phosphate, pH 7.4 containing 150mM Sodium chloride
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Short-term (up to 7 days) store at 2 to 8 degree C. Long term, aliquot and store at <-20 degree C. If aliquoted for long term storage, fill volume should be equal to or greater than 50% of the nominal fill volume of the vial used. Avoid repeated freeze/th

Product Categories/Family for anti-AFP antibody

Monoclonal Antibodies for Cancer Research

NCBI and Uniprot Product Information

• NCBI GI #: 178236
• NCBI GeneID: 174
• UniProt Accession #: P02771; B2RBU3
• Molecular Weight: 68,678 Da
• NCBI Official Full Name: alpha-fetoprotein
• NCBI Official Synonym Full Names: alpha-fetoprotein
• NCBI Official Symbol: AFP
• NCBI Official Synonym Symbols: FETA; HPAFP; AFP
• NCBI Protein Information: alpha-fetoprotein
• UniProt Protein Name: Alpha-fetoprotein
• Protein Family: Alpha-fetoprotein
• UniProt Gene Name: AFP
• UniProt Synonym Gene Names: HPAFP
• UniProt Entry Name: FETA_HUMAN

NCBI Description

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular location: Secreted.

Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.

Developmental stage: Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

Post-translational modification: Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated.

Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.