Testing Data
anti-Human Epidermal Growth Factor Receptor (EGFR), phosphorylated Tyr 1045 + Positive Monoclonal Antibody | anti-EGFR antibody
MAb to EGFR, pTyr 1045
Source: Cell Culture
Recognizes EGFR phosphorylated at Tyrosine 1045. Epitope: Phosphotyrosine pY1045 L Q R pY S S D. Reacts with Human and mouse.
Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium chloride
Immunoprecipitation: use at 1-10
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq]
Uniprot Description
Function: Cell surface receptor for EGF, but also for other members of the EGF family, such as TGF-alpha, BTC/betacellulin, AREGAREGB/amphiregulin, HBEGF, GP30 and vaccinia virus growth factor. Ligand binding triggers a conformation change, leading to activation of the kinase and subsequent phosphorylation of down-stream protein kinases. Is involved in the control of cell growth, proliferation and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65Isoform 2 may act as an antagonist of EGF action. Ref.33 Ref.54 Ref.55 Ref.56 Ref.60 Ref.63 Ref.65
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Ref.56 Ref.59 Ref.60 Ref.61 Ref.62 Ref.63
Subunit structure: EGF binding triggers dimerization of the receptor and promotes autophosphorylation. The activate receptor dimer binds one EGF molecule. Heterodimer with ERBB2. Interacts with ERRFI1. Interaction with ERRFI1 inhibits dimerization of the kinase domain and autophosphorylation. Binds RIPK1. Interacts (via autophosphorylated C-terminal tail) with CBL. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. Interacts (autophosphorylated form) with PIK3C2B; the interaction may be indirect. Interacts with PELP1. Binds MUC1. Interacts with AP2M1. Interacts with GAB2. Interacts with COPG, upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with TNK2; this interaction is dependent on EGF stimulation and kinase activity of EGFR. Ref.26 Ref.28 Ref.31 Ref.32 Ref.33 Ref.37 Ref.41 Ref.44 Ref.52 Ref.54 Ref.55 Ref.60 Ref.62 Ref.63 Ref.64 Ref.65
Subcellular location: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes. Ref.41 Ref.52 Ref.54 Ref.63Isoform 2: Secreted Ref.41 Ref.52 Ref.54 Ref.63.
Tissue specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.40
Post-translational modification: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.17 Ref.25 Ref.30 Ref.34 Ref.35 Ref.36 Ref.38 Ref.39 Ref.42 Ref.43 Ref.45 Ref.46 Ref.47 Ref.48 Ref.50 Ref.51 Ref.59 Ref.60 Ref.62 Ref.63Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
Involvement in disease: Defects in EGFR are associated with lung cancer (LNCR) [
MIM:211980].
Miscellaneous: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.Contains 1 protein kinase domain.
Research Articles on EGFR
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Product Notes
The EGFR egfr (Catalog #AAA311322) is an Antibody produced from Host: Mouse Source: Cell Culture and is intended for research purposes only. The product is available for immediate purchase. The MAb to EGFR, pTyr 1045 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Epidermal Growth Factor Receptor (EGFR), phosphorylated Tyr 1045 + Positive can be used in a range of immunoassay formats including, but not limited to, EIA/ELISA, Western Blot. Immunoblotting: 0.5ug/ml for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium chloride Immunoprecipitation: use at 1-10. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor Receptor (EGFR), phosphorylated Tyr 1045 + Positive, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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