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FCM (Flow Cytometry) (Flow cytometric analysis of PC-3M cells with Galectin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Rabbit Galectin 8 Monoclonal Antibody | anti-LGALS8 antibody

Galectin 8 Antibody

Gene Names
LGALS8; Gal-8; PCTA1; PCTA-1; Po66-CBP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
Galectin 8; Monoclonal Antibody; Galectin 8 Antibody; Gal 8 antibody; Gal-8 antibody; Gal8 antibody; Galectin-8 antibody; galectin-8g antibody; Lectin galactoside binding soluble 8 antibody; LEG8_HUMAN antibody; LGAL S8 antibody; Lgals8 antibody; PCTA 1 antibody; PCTA-1 antibody; PCTA1 antibody; Po66 carbohydrate binding protein antibody; Po66 carbohydrate-binding protein antibody; Po66 CBP antibody; Po66-CBP antibody; Prostate carcinoma tumor antigen 1 antibody; anti-LGALS8 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB85-35
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-LGALS8 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC/IF: 1:400-1:800
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of PC-3M cells with Galectin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

FCM (Flow Cytometry) (Flow cytometric analysis of PC-3M cells with Galectin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining Galectin 8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Galectin 8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Galectin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Galectin 8 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.)

WB (Western Blot) (Western blot analysis of Galectin 8 on mouse testis and rat testis tissue lysates using anti-Galectin 8 antibody at 1/500 dilution.)
Related Product Information for anti-LGALS8 antibody
Several proteins have been identified as specific markers of prostate cancer, and they may be useful as identification indicators. PSA, prostate specific antigen, is the classical indicator for transformed prostate tissue; however, in addition to being up-regulated in prostate cancer, PSA is also up-regulated in non-malignant conditions, such as benign prostatic hyperplasia prostate. Galectin-8, also known as prostate-specific membrane antigen (PCTA-1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates bothe cell-cell and cell-matrix interactions in a manner similar to the selectin subgroup of C-type lectins.
Product Categories/Family for anti-LGALS8 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,808 Da
NCBI Official Full Name
galectin-8 isoform a
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 8
NCBI Official Symbol
LGALS8
NCBI Official Synonym Symbols
Gal-8; PCTA1; PCTA-1; Po66-CBP
NCBI Protein Information
galectin-8; galectin 8; galectin-8g; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1
UniProt Protein Name
Galectin-8
UniProt Gene Name
LGALS8
UniProt Synonym Gene Names
Gal-8; Po66-CBP; PCTA-1
UniProt Entry Name
LEG8_HUMAN

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Product Notes

The LGALS8 lgals8 (Catalog #AAA30457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Galectin 8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin 8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC/IF: 1:400-1:800 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the LGALS8 lgals8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galectin 8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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