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Western Blot (WB) (Western blot (WB) analysis of C/EBP epsilon (Phospho-T74) polyclonal antibody at 1:500 dilutionLaneA: Hela whole cell lysateLaneB: The Liver tissue lysate of RatLaneC: Raw264.7 whole cell lysateLaneD: The Liver tissue lysate of Rat)

Rabbit C/EBP epsilon Polyclonal Antibody | anti-C/EBPepsilon antibody

C/EBP epsilon (Phospho-T74) polyclonal antibody

Gene Names
CEBPE; CRP1; C/EBP-epsilon
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
C/EBP epsilon; Polyclonal Antibody; C/EBP epsilon (Phospho-T74) polyclonal antibody; CCAAT/enhancer-binding protein epsilon; CEBPE; C/EBPepsilon; anti-C/EBPepsilon antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
C/EBP epsilon (Phospho-T74) polyclonal antibody detects endogenous levels of C/EBP epsilon protein only when phosphorylated at Thr74.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-C/EBPepsilon antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
Immunogen
Synthetic phosphopeptide derived from human C/EBP epsilon around the phosphorylation site of Threonine 74
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of C/EBP epsilon (Phospho-T74) polyclonal antibody at 1:500 dilutionLaneA: Hela whole cell lysateLaneB: The Liver tissue lysate of RatLaneC: Raw264.7 whole cell lysateLaneD: The Liver tissue lysate of Rat)

Western Blot (WB) (Western blot (WB) analysis of C/EBP epsilon (Phospho-T74) polyclonal antibody at 1:500 dilutionLaneA: Hela whole cell lysateLaneB: The Liver tissue lysate of RatLaneC: Raw264.7 whole cell lysateLaneD: The Liver tissue lysate of Rat)
Related Product Information for anti-C/EBPepsilon antibody
C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally-differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma, C/EBP delta and C/EBP epsilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,603 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein epsilon
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), epsilon
NCBI Official Symbol
CEBPE
NCBI Official Synonym Symbols
CRP1; C/EBP-epsilon
NCBI Protein Information
CCAAT/enhancer-binding protein epsilon; c/EBP epsilon
UniProt Protein Name
CCAAT/enhancer-binding protein epsilon
UniProt Gene Name
CEBPE
UniProt Synonym Gene Names
C/EBP epsilon
UniProt Entry Name
CEBPE_HUMAN

NCBI Description

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

C/EBP-epsilon: C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Belongs to the bZIP family. C/EBP subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm

Molecular Function: protein homodimerization activity; protein heterodimerization activity

Biological Process: macrophage differentiation; transcription, DNA-dependent; cytokine biosynthetic process; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; defense response; phagocytosis

Disease: Specific Granule Deficiency

Research Articles on C/EBPepsilon

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Product Notes

The C/EBPepsilon cebpe (Catalog #AAA3015029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C/EBP epsilon (Phospho-T74) polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP epsilon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the C/EBPepsilon cebpe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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