Rabbit SGK1 Polyclonal Antibody | anti-SGK1 antibody

SGK1 (Phospho-S78) polyclonal antibody

Cat. Num. AAA3015024

• Gene Names: SGK1; SGK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: SGK1; Polyclonal Antibody; SGK1 (Phospho-S78) polyclonal antibody; Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; anti-SGK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SGK1 (Phospho-S78) polyclonal antibody detects endogenous levels of SGK1 protein only when phosphorylated at Ser78.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SGK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of Serine 78
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of SGK1 (Phospho-S78) polyclonal antibody at 1:500 dilutionLaneA: Zebrafish)

Related Product Information for anti-SGK1 antibody

Serum- and glucocorticoid-regulated kinase (SGK), also known as SGK1, is a serine/threonine protein kinase and a member of the "AGC" subfamily, which includes protein kinases A, G, and C. SGK plays an important role in activating certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK contains a catalytic domain, which is most similar to Akt1.

NCBI and Uniprot Product Information

• NCBI GI #: 219521876
• NCBI GeneID: 6446
• NCBI Accession #: NP_001137148.1
• NCBI GenBank Nucleotide #: NM_001143676.1
• UniProt Accession #: O00141; Q5TCN2; Q5TCN3; Q5TCN4; Q5VY65; Q9UN56; B7UUP7; B7UUP8; B7UUP9; B7Z5B2; E1P583
• Molecular Weight: 48,942 Da
• NCBI Official Full Name: serine/threonine-protein kinase Sgk1 isoform 2
• NCBI Official Synonym Full Names: serum/glucocorticoid regulated kinase 1
• NCBI Official Symbol: SGK1
• NCBI Official Synonym Symbols: SGK
• NCBI Protein Information: serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase Sgk1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SGK1
• UniProt Synonym Gene Names: SGK
• UniProt Entry Name: SGK1_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Protein kinase, AGC; EC 2.7.11.1; AGC group; SGK family

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: apoptosis; sodium ion transport; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation; regulation of apoptosis; regulation of transcription factor activity; long-term memory; regulation of catalytic activity; regulation of blood pressure; response to stress; regulation of cell growth; transmembrane transport; response to DNA damage stimulus