Rabbit CCR7 Monoclonal Antibody | anti-CCR7 antibody

CCR7 Rabbit Monoclonal Antibody

Cat. Num. AAA3014072

• Gene Names: CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: Protein A affinity purified
• Synonyms: CCR7; Monoclonal Antibody; CCR7 Rabbit Monoclonal Antibody; C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR-7; BLR2; CDw197; Epstein-Barr virus-induced G-protein coupled receptor 1; EBI1; EBV-induced G-protein coupled receptor 1; MIP-3 beta receptor; CD197; CKR7 CMKBR7; EVI1; anti-CCR7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of CCR7 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 378

• Applicable Applications for anti-CCR7 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; ICC: 1:50-1:200; IF: 1:50-1:100; IHC: 1:50-1:200
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CCR7 Rabbit mAb at 1:1000 dilutionLane1:MCF7 whole cell lysateLane2:PC12 whole cell lysateLane3:Daudi whole cell lysateLane4:Jurkat whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CCR7 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining CCR7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR7 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining CCR7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Related Product Information for anti-CCR7 antibody

C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7 and the Duffy blood group antigen. Each of these receptors are G protein-coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. Termed a "co-receptor", CKR-5, along with CD4, has been shown to be a major receptor for HIV. CKR-5 tends to associate with macrophagetropic viruses, such as macrophage tropic HIV-1, while CKR-2B and CKR-3 bind a minority of viruses.

NCBI and Uniprot Product Information

• NCBI GI #: 4502641
• NCBI GeneID: 1236
• NCBI Accession #: NP_001829.1
• NCBI GenBank Nucleotide #: NP_001829.1
• UniProt Accession #: P32248
• Molecular Weight: ~ 43kDa
• NCBI Official Full Name: C-C chemokine receptor type 7 isoform a
• NCBI Official Synonym Full Names: C-C motif chemokine receptor 7
• NCBI Official Symbol: CCR7
• NCBI Official Synonym Symbols: BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• NCBI Protein Information: C-C chemokine receptor type 7
• UniProt Protein Name: C-C chemokine receptor type 7
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR7
• UniProt Synonym Gene Names: CMKBR7; EBI1; EVI1; C-C CKR-7; CC-CKR-7; CCR-7; EBI1
• UniProt Entry Name: CCR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q12-q21.2

Cellular Component: cell surface; integral to membrane; plasma membrane; intracellular; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; C-C chemokine receptor activity

Biological Process: positive regulation of cell adhesion; positive regulation of interleukin-12 production; positive regulation of dendritic cell antigen processing and presentation; positive regulation of JNK cascade; response to lipopolysaccharide; elevation of cytosolic calcium ion concentration; positive regulation of T cell receptor signaling pathway; positive regulation of humoral immune response; dendritic cell chemotaxis; establishment of T cell polarity; inflammatory response; positive regulation of cell-matrix adhesion; positive regulation of filopodium formation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of hypersensitivity; positive regulation of phosphoinositide 3-kinase activity; negative thymic T cell selection; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; positive regulation of pseudopodium formation; myeloid dendritic cell chemotaxis; positive regulation of actin filament polymerization; regulation of interferon-gamma production; positive regulation of protein kinase activity; release of sequestered calcium ion into cytosol; ruffle organization and biogenesis; regulation of interleukin-1 beta secretion; immune response